Display options
Cite
Caldwell JB, Sparrow LG. Partial purification and characterization of two Peptide hydrolases from pea seeds. Plant Physiol. 1976;57(5):795-8doi: 10.1104/pp.57.5.795.
Caldwell, J. B., & Sparrow, L. G. (1976). Partial purification and characterization of two Peptide hydrolases from pea seeds. Plant physiology, 57(5), 795-8. https://doi.org/10.1104/pp.57.5.795
Caldwell, J B, and Sparrow, L G. "Partial purification and characterization of two Peptide hydrolases from pea seeds." Plant physiology vol. 57,5 (1976): 795-8. doi: https://doi.org/10.1104/pp.57.5.795
Caldwell JB, Sparrow LG. Partial purification and characterization of two Peptide hydrolases from pea seeds. Plant Physiol. 1976 May;57(5):795-8. doi: 10.1104/pp.57.5.795. PMID: 16659571; PMCID: PMC542119.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1976 May;57(5):795-8. doi: 10.1104/pp.57.5.795.

Partial purification and characterization of two Peptide hydrolases from pea seeds.

Plant physiology

J B Caldwell, L G Sparrow

Affiliations

  1. Division of Protein Chemistry, Commonwealth Scientific and Industrial Research Organization, Parkville (Melbourne), Victoria 3052, Australia.

PMID: 16659571 PMCID: PMC542119 DOI: 10.1104/pp.57.5.795

Abstract

Two peptide hydrolases have been found in pea seeds (Pisum sativum var. Greenfeast) and extensively purified by ion exchange chromatography using benzoyl-dl-arginine-p-nitroanilide as substrate. The enzymes which both have molecular weights of 65,000 can be separated by anion exchange chromatography but are otherwise virtually identical in the properties tested. They did not hydrolyze several common protease substrates but readily hydrolyzed small peptides containing basic amino acids on the carboxyl side of these residues. They are completely inhibited by diisopropylfluorophosphate and are inhibited to varying extents by thiol reagents.

References

  1. Eur J Biochem. 1969 Jan;7(2):193-9 - PubMed
  2. Biochim Biophys Acta. 1972 May 18;263(3):673-9 - PubMed
  3. J Biol Chem. 1963 Feb;238:622-7 - PubMed
  4. Can J Biochem. 1972 Dec;50(12):1297-310 - PubMed
  5. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
  6. Arch Biochem Biophys. 1961 Nov;95:271-8 - PubMed
  7. J Biol Chem. 1968 Jun 10;243(11):2882-9 - PubMed
  8. Biokhimiia. 1973 Sep-Oct;38(5):964-70 - PubMed
  9. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  10. Comp Biochem Physiol B. 1976;53(2):187-90 - PubMed
  11. Plant Physiol. 1971 Sep;48(3):278-81 - PubMed
  12. Arch Biochem Biophys. 1957 Mar;67(1):10-5 - PubMed
  13. Biochem J. 1974 Jul;141(1):113-8 - PubMed
  14. Anal Biochem. 1973 Dec;56(2):596-600 - PubMed
  15. J Sci Food Agric. 1971 Feb;22(2):79-82 - PubMed
  16. Biochem J. 1960 Oct;77:149-63 - PubMed
  17. Can J Biochem. 1974 Oct;52(10):903-10 - PubMed
  18. Biochem J. 1964 May;91(2):222-33 - PubMed

Publication Types