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Appl Opt. 2006 Mar 20;45(9):1964-71. doi: 10.1364/ao.45.001964.

High-resolution angle-resolved measurements of light scattered at small angles by red blood cells in suspension.

Applied optics

Ioan Turcu, Cristian V L Pop, Silvia Neamtu

Affiliations

  1. National Institute for Research and Development of Isotopic and Molecular Technologies, P.O. Box 700, RO-400293 Cluj-Napoca, Romania. [email protected]

PMID: 16579566 DOI: 10.1364/ao.45.001964

Abstract

Red blood cells (RBCs) scatter light mainly in the forward direction, where the scattering phase function has a narrow peak. We performed an experimental investigation into the angular distribution of light scattered by blood in the small-angle domain. A highly diluted suspension of RBCs (hematocrits in the range 5 x 10(-5)-10(-2)) was illuminated with a He-Ne laser with 633 nm wavelength. We focused our research on two main topics: the scattering efficiency of the RBCs given by the mean scattering cross section and the scattering anisotropy obtained from the angular distribution of the scattered photons. The collimated beam transmission and the angular distribution of scattered light were measured and compared with the predictions of the effective phase function model. The RBCs' mean scattering cross section and scattering anisotropy were obtained by fitting of the experimental data.

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