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Ohyama K, Pelcher LE, Horn D. A rapid, simple method for nuclei isolation from plant protoplasts. Plant Physiol. 1977;60(2):179-81doi: 10.1104/pp.60.2.179.
Ohyama, K., Pelcher, L. E., & Horn, D. (1977). A rapid, simple method for nuclei isolation from plant protoplasts. Plant physiology, 60(2), 179-81. https://doi.org/10.1104/pp.60.2.179
Ohyama, K, et al. "A rapid, simple method for nuclei isolation from plant protoplasts." Plant physiology vol. 60,2 (1977): 179-81. doi: https://doi.org/10.1104/pp.60.2.179
Ohyama K, Pelcher LE, Horn D. A rapid, simple method for nuclei isolation from plant protoplasts. Plant Physiol. 1977 Aug;60(2):179-81. doi: 10.1104/pp.60.2.179. PMID: 16660054; PMCID: PMC542574.
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Plant Physiol. 1977 Aug;60(2):179-81. doi: 10.1104/pp.60.2.179.

A rapid, simple method for nuclei isolation from plant protoplasts.

Plant physiology

K Ohyama, L E Pelcher, D Horn

Affiliations

  1. National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Saskatchewan S7N 0W9.

PMID: 16660054 PMCID: PMC542574 DOI: 10.1104/pp.60.2.179

Abstract

A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.

References

  1. Plant Physiol. 1970 Apr;45(4):372-5 - PubMed
  2. Plant Physiol. 1972 Sep;50(3):319-21 - PubMed
  3. Plant Physiol. 1975 Jul;56(1):78-82 - PubMed
  4. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1213-9 - PubMed

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