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Garcia RE, Mudd JB. Identification of an acyl donor in steryl ester biosynthesis by enzyme preparations from spinach leaves. Plant Physiol. 1978;62(3):348-53doi: 10.1104/pp.62.3.348.
Garcia, R. E., & Mudd, J. B. (1978). Identification of an acyl donor in steryl ester biosynthesis by enzyme preparations from spinach leaves. Plant physiology, 62(3), 348-53. https://doi.org/10.1104/pp.62.3.348
Garcia, R E, and Mudd, J B. "Identification of an acyl donor in steryl ester biosynthesis by enzyme preparations from spinach leaves." Plant physiology vol. 62,3 (1978): 348-53. doi: https://doi.org/10.1104/pp.62.3.348
Garcia RE, Mudd JB. Identification of an acyl donor in steryl ester biosynthesis by enzyme preparations from spinach leaves. Plant Physiol. 1978 Sep;62(3):348-53. doi: 10.1104/pp.62.3.348. PMID: 16660515; PMCID: PMC1092124.
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Plant Physiol. 1978 Sep;62(3):348-53. doi: 10.1104/pp.62.3.348.

Identification of an acyl donor in steryl ester biosynthesis by enzyme preparations from spinach leaves.

Plant physiology

R E Garcia, J B Mudd

Affiliations

  1. Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92521.

PMID: 16660515 PMCID: PMC1092124 DOI: 10.1104/pp.62.3.348

Abstract

A pathway for steryl ester biosynthesis in acetone powder preparations from spinach (Spinacia oleracea L.) leaves has been elucidated; free sterol and 1,2-diglyceride were the substrates. Although animals synthesize cholesteryl esters by three distinct biosynthetic pathways, none of these pathways utilizes 1,2-diglyceride as an acyl donor. Phosphatidylcholine, phosphatidic acid, triglyceride, 1,3-diglyceride, 1-monoglyceride, free fatty acid, and fatty acyl-CoA were not acyl donors for spinach leaf steryl ester biosynthesis in our assay system. The unstable 2 isomer of monoglyceride was not tested. It is possible that 1,2-diglyceride and 2-monoglyceride were both acyl donors for spinach leaf steryl ester biosynthesis. Acyl-labeled phosphatidylcholine and acyl-labeled phosphatidylethanolamine were rapidly degraded by acetone powder preparations to 1,2-diglyceride via phosphatidic acid. The 1,2-diglycerides were slowly metabolized to monoglycerides, triglycerides, free fatty acids, and steryl esters. The monoglycerides were rapidly degraded to free fatty acids and glycerol.

References

  1. Plant Physiol. 1965 Mar;40:235-43 - PubMed
  2. Can J Biochem Physiol. 1959 Aug;37(8):911-7 - PubMed
  3. J Biol Chem. 1966 Feb 10;241(3):720-6 - PubMed
  4. Biochemistry. 1967 Sep;6(9):2705-17 - PubMed
  5. J Biol Chem. 1969 Apr 10;244(7):1937-45 - PubMed
  6. Can J Biochem Physiol. 1954 Sep;32(5):571-83 - PubMed
  7. Plant Physiol. 1973 Jul;52(1):50-3 - PubMed
  8. Biochem J. 1969 May;112(5):787-94 - PubMed
  9. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  10. J Biol Chem. 1964 May;239:1335-45 - PubMed
  11. J Biol Chem. 1973 Apr 10;248(7):2317-22 - PubMed

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