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Tully RE, Beevers H. Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination. Plant Physiol. 1978;62(5):746-50doi: 10.1104/pp.62.5.746.
Tully, R. E., & Beevers, H. (1978). Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination. Plant physiology, 62(5), 746-50. https://doi.org/10.1104/pp.62.5.746
Tully, R E, and Beevers, H. "Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination." Plant physiology vol. 62,5 (1978): 746-50. doi: https://doi.org/10.1104/pp.62.5.746
Tully RE, Beevers H. Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination. Plant Physiol. 1978 Nov;62(5):746-50. doi: 10.1104/pp.62.5.746. PMID: 16660598; PMCID: PMC1092213.
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Plant Physiol. 1978 Nov;62(5):746-50. doi: 10.1104/pp.62.5.746.

Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination.

Plant physiology

R E Tully, H Beevers

Affiliations

  1. Division of Natural Sciences, University of California, Santa Cruz, California 95064.

PMID: 16660598 PMCID: PMC1092213 DOI: 10.1104/pp.62.5.746

Abstract

The endosperm of castor bean seeds (Ricinus communis L.) contains two -SH-dependent aminopeptidases, one hydrolyzing l-leucine-beta-naphthylamide optimally at pH 7.0, and the other hydrolyzing l-proline-beta-naphthylamide optimally at pH 7.5. After germination the endosperm contains in addition an -SH-dependent hemoglobin protease, a serine-dependent carboxypeptidase, and at least two -SH-dependent enzymes hydrolyzing the model substrate alpha-N-benzoyl-dl-arginine-beta-naphthylamide (BANA). The carboxypeptidase is active on a variety of N-carbobenzoxy dipeptides, especially N-carbobenzoxy-L-phenylalanine-l-alanine and N-carbobenzoxy-l-tyrosine-l-leucine. The pH optima for the protease, carboxypeptidase, and BANAase acivities are 3.5 to 4.0, 5.0 to 5.5, and 6 to 8, respectively.The two aminopeptidases increased about 4-fold in activity during the first 4 days of growth, concurrent with the period of rapid depletion of storage protein. Activities then declined as the endosperm senesced, but were still evident after 6 days. Senescence was complete by day 7 to 8. Hemoglobin protease, carboxypeptidase, and BANAase activities appeared in the endosperm at day 2 to 3, and reached peak activity at day 5 to 6.The data indicate that the aminopeptidases are involved in the early mobilization of endosperm storage protein, whereas protease, carboxypeptidase, and BANAase may take part in later turnover and/or senescence.

References

  1. Eur J Biochem. 1969 Jan;7(2):193-9 - PubMed
  2. Plant Physiol. 1975 May;55(5):809-14 - PubMed
  3. Plant Physiol. 1975 Jun;55(6):1031-7 - PubMed
  4. Eur J Biochem. 1970 Nov;17(1):13-8 - PubMed
  5. Plant Physiol. 1976 May;57(5):795-8 - PubMed
  6. Plant Physiol. 1976 Dec;58(6):710-6 - PubMed
  7. Eur J Biochem. 1970 Nov;17(1):4-12 - PubMed
  8. Plant Physiol. 1976 Dec;58(6):703-9 - PubMed
  9. Biochem J. 1974 Jul;141(1):113-8 - PubMed
  10. Plant Physiol. 1976 Oct;58(4):516-20 - PubMed
  11. Plant Physiol. 1973 Dec;52(6):671-3 - PubMed
  12. Plant Physiol. 1974 Dec;54(6):925-30 - PubMed
  13. J Biochem. 1975 Jun;77(6):1297-303 - PubMed
  14. J Biol Chem. 1972 Aug 25;247(16):5034-40 - PubMed
  15. Arch Biochem Biophys. 1957 Mar;67(1):10-5 - PubMed
  16. Arch Biochem Biophys. 1971 Aug;145(2):633-42 - PubMed
  17. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed

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