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Perlin DS, Spanswick RM. Labeling and isolation of plasma membranes from corn leaf protoplasts. Plant Physiol. 1980;65(6):1053-7doi: 10.1104/pp.65.6.1053.
Perlin, D. S., & Spanswick, R. M. (1980). Labeling and isolation of plasma membranes from corn leaf protoplasts. Plant physiology, 65(6), 1053-7. https://doi.org/10.1104/pp.65.6.1053
Perlin, D S, and Spanswick, R M. "Labeling and isolation of plasma membranes from corn leaf protoplasts." Plant physiology vol. 65,6 (1980): 1053-7. doi: https://doi.org/10.1104/pp.65.6.1053
Perlin DS, Spanswick RM. Labeling and isolation of plasma membranes from corn leaf protoplasts. Plant Physiol. 1980 Jun;65(6):1053-7. doi: 10.1104/pp.65.6.1053. PMID: 16661329; PMCID: PMC440479.
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Plant Physiol. 1980 Jun;65(6):1053-7. doi: 10.1104/pp.65.6.1053.

Labeling and isolation of plasma membranes from corn leaf protoplasts.

Plant physiology

D S Perlin, R M Spanswick

Affiliations

  1. Section of Botany, Genetics and Development, Division of Biological Sciences, Plant Science Building, Cornell University, Ithaca, New York 14853.

PMID: 16661329 PMCID: PMC440479 DOI: 10.1104/pp.65.6.1053

Abstract

A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [(125)I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mitochondrial markers, whereas markers for the endoplasmic reticulum and golgi indicated minimal contamination. The highly enriched plasma membrane fraction contained a Mg(2+)-dependent, K(+)-stimulated ATPase with a pH optimum near neutrality. The position of the membranes on sucrose density gradients indicates that the plasma membranes have characteristics similar to other plasma membrane fractions.

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