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Graf RN, Wax A. Nuclear morphology measurements using Fourier domain low coherence interferometry. Opt Express. 2005;13(12):4693-4698doi: 10.1364/opex.13.004693.
Graf, R. N., & Wax, A. (2005). Nuclear morphology measurements using Fourier domain low coherence interferometry. Optics express, 13(12), 4693-4698. https://doi.org/10.1364/opex.13.004693
Graf, Robert N, and Wax, Adam. "Nuclear morphology measurements using Fourier domain low coherence interferometry." Optics express vol. 13,12 (2005): 4693-4698. doi: https://doi.org/10.1364/opex.13.004693
Graf RN, Wax A. Nuclear morphology measurements using Fourier domain low coherence interferometry. Opt Express. 2005 Jun 13;13(12):4693-4698. doi: 10.1364/opex.13.004693. PMID: 16429608; PMCID: PMC1317105.
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Opt Express. 2005 Jun 13;13(12):4693-4698. doi: 10.1364/opex.13.004693.

Nuclear morphology measurements using Fourier domain low coherence interferometry.

Optics express

Robert N Graf, Adam Wax

Affiliations

  1. Department of Biomedical Engineering and the Fitzpatrick Center for Photonics Duke University, Durham, NC 27708 [email protected].

PMID: 16429608 PMCID: PMC1317105 DOI: 10.1364/opex.13.004693

Abstract

We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile.

References

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