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Uchimiya H. Parameters influencing the liposome-mediated insertion of fluorescein diacetate into plant protoplasts. Plant Physiol. 1981;67(4):629-32doi: 10.1104/pp.67.4.629.
Uchimiya, H. (1981). Parameters influencing the liposome-mediated insertion of fluorescein diacetate into plant protoplasts. Plant physiology, 67(4), 629-32. https://doi.org/10.1104/pp.67.4.629
Uchimiya, H. "Parameters influencing the liposome-mediated insertion of fluorescein diacetate into plant protoplasts." Plant physiology vol. 67,4 (1981): 629-32. doi: https://doi.org/10.1104/pp.67.4.629
Uchimiya H. Parameters influencing the liposome-mediated insertion of fluorescein diacetate into plant protoplasts. Plant Physiol. 1981 Apr;67(4):629-32. doi: 10.1104/pp.67.4.629. PMID: 16661727; PMCID: PMC425745.
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Plant Physiol. 1981 Apr;67(4):629-32. doi: 10.1104/pp.67.4.629.

Parameters influencing the liposome-mediated insertion of fluorescein diacetate into plant protoplasts.

Plant physiology

H Uchimiya

Affiliations

  1. Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan.

PMID: 16661727 PMCID: PMC425745 DOI: 10.1104/pp.67.4.629

Abstract

Maximum uptake of liposome-encapsulated fluorescein diacetate by Daucus carota protoplasts was observed when 6 x 10(6) protoplasts per milliliter were incubated with 2.4 x 10(7) liposomes per milliliter for 1 hour. In the case of Nicotiana glutinosa protoplasts, optimum ratio of protoplasts to liposomes was 1:10, where 2.3 x 10(5) protoplasts per milliliter were provided. Neutral and positive liposomes were found to be efficient vehicles to transfer their contents into plant protoplasts. When protoplasts treated with liposomes were cultured in a synthetic medium for 1 week, 20% resumed cell divisions.

References

  1. Plant Physiol. 1974 Dec;54(6):936-44 - PubMed
  2. Plant Physiol. 1979 Dec;64(6):959-62 - PubMed
  3. Methods Cell Biol. 1976;14:33-71 - PubMed

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