Display options
Cite
Larsen PO, Cornwell KL, Gee SL, et al. Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2). Plant Physiol. 1981;68(2):292-9doi: 10.1104/pp.68.2.292.
Larsen, P. O., Cornwell, K. L., Gee, S. L., & Bassham, J. A. (1981). Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2). Plant physiology, 68(2), 292-9. https://doi.org/10.1104/pp.68.2.292
Larsen, P O, et al. "Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2)." Plant physiology vol. 68,2 (1981): 292-9. doi: https://doi.org/10.1104/pp.68.2.292
Larsen PO, Cornwell KL, Gee SL, Bassham JA. Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2). Plant Physiol. 1981 Aug;68(2):292-9. doi: 10.1104/pp.68.2.292. PMID: 16661904; PMCID: PMC427478.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1981 Aug;68(2):292-9. doi: 10.1104/pp.68.2.292.

Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2).

Plant physiology

P O Larsen, K L Cornwell, S L Gee, J A Bassham

Affiliations

  1. Laboratory of Chemical Biodynamics, University of California, Berkeley, California 94720.

PMID: 16661904 PMCID: PMC427478 DOI: 10.1104/pp.68.2.292

Abstract

Isolated cells from leaves of Spinacia oleracea have been maintained in a state capable of high rates of photosynthetic CO(2) fixation for more than 60 hours. The incorporation of (14)CO(2) under saturating CO(2) conditions into carbohydrates, carboxylic acids, and amino acids, and the effect of ammonia on this incorporation have been studied. Total incorporation, specific radioactivity, and pool size have been determined as a function of time for most of the protein amino acids and for gamma-aminobutyric acid. The measurements of specific radio-activities and of the approaches to (14)C "saturation" of some amino acids indicate the presence and relative sizes of metabolically active and passive pools of these amino acids.Added ammonia decreased carbon fixation into carbohydrates and increased fixation into carboxylic acids and amino acids. Different amino acids were, however, affected in different and highly specific ways. Ammonia caused large stimulatory effects in incorporation of (14)C into glutamine (a factor of 21), aspartate, asparagine, valine, alanine, arginine, and histidine. No effect or slight decreases were seen in glycine, serine, phenylalanine, and tyrosine labeling. In the case of glutamate, (14)C labeling decreased, but specific radioactivity increased. The production of labeled gamma-aminobutyric acid was virtually stopped by ammonia.The results indicate that added ammonia stimulates the reactions mediated by pyruvate kinase and phosphoenolpyruvate carboxylase, as seen with other plant systems. The data on the effects of added ammonia on total labeling, pool sizes, and specific radioactivities of several amino acids provides a number of indications about the intracellular sites of principal synthesis from carbon skeletons of these amino acids and the selective nature of effects of increased intracellular ammonia concentration on such synthesis.

References

  1. Plant Physiol. 1977 Nov;60(5):739-42 - PubMed
  2. Plant Physiol. 1978 Aug;62(2):210-4 - PubMed
  3. Anal Biochem. 1979 Jul 1;96(1):45-55 - PubMed
  4. Biochim Biophys Acta. 1972 Mar 16;256(3):656-69 - PubMed
  5. Biochim Biophys Acta. 1970 Jun 30;205(3):401-8 - PubMed
  6. Plant Physiol. 1979 Aug;64(2):263-8 - PubMed
  7. Plant Physiol. 1949 Jan;24(1):1-15 - PubMed
  8. Plant Physiol. 1980 Jun;65(6):1166-72 - PubMed
  9. Plant Physiol. 1973 Jul;52(1):17-22 - PubMed
  10. Symp Soc Exp Biol. 1973;27:513-29 - PubMed
  11. J Chromatogr. 1971 Jan 20;54(2):215-23 - PubMed
  12. Plant Physiol. 1977 Nov;60(5):775-8 - PubMed
  13. Anal Biochem. 1978 Mar;85(1):71-8 - PubMed

Publication Types