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Waters SP, Noble ER, Dalling MJ. Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves. Plant Physiol. 1982;69(3):575-9doi: 10.1104/pp.69.3.575.
Waters, S. P., Noble, E. R., & Dalling, M. J. (1982). Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves. Plant physiology, 69(3), 575-9. https://doi.org/10.1104/pp.69.3.575
Waters, S P, et al. "Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves." Plant physiology vol. 69,3 (1982): 575-9. doi: https://doi.org/10.1104/pp.69.3.575
Waters SP, Noble ER, Dalling MJ. Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves. Plant Physiol. 1982 Mar;69(3):575-9. doi: 10.1104/pp.69.3.575. PMID: 16662252; PMCID: PMC426257.
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Plant Physiol. 1982 Mar;69(3):575-9. doi: 10.1104/pp.69.3.575.

Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves.

Plant physiology

S P Waters, E R Noble, M J Dalling

Affiliations

  1. Plant Sciences Section, School of Agriculture and Forestry, University of Melbourne, Parkville, Victoria 3052, Australia.

PMID: 16662252 PMCID: PMC426257 DOI: 10.1104/pp.69.3.575

Abstract

Protoplasts from 8- to 9-day-old wheat (Triticum aestivum L.) leaves were used to isolate organelles which were examined for their contents of peptide hydrolase enzymes and, in the case of vacuoles, other acid hydrolases. High yields of intact chloroplasts were obtained using both equilibrium density gradient centrifugation and velocity sedimentation centrifugation on sucrose-sorbitol gradients. Aminopeptidase activity was found to be distributed, in approximately equal proportions, between the chloroplasts and cytoplasm. Leucyltyrosine dipeptidase was mainly found in the cytoplasm, although about 27% was associated with the chloroplasts. Vacuoles shown to be free from Cellulysin contamination contained all of the protoplast carboxypeptidase and hemoglobin-degrading activities. The acid hydrolases, phosphodiesterase, acid phosphatase, alpha-mannosidase, and beta-N-acetylglucosamidase were found in the vacuole to varying degrees, but no beta-glucosidase was localized in the vacuole.

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