Display options
Cite
Atkins CA, Ritchie A, Rowe PB, et al. De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.). Plant Physiol. 1982;70(1):55-60doi: 10.1104/pp.70.1.55.
Atkins, C. A., Ritchie, A., Rowe, P. B., McCairns, E., & Sauer, D. (1982). De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.). Plant physiology, 70(1), 55-60. https://doi.org/10.1104/pp.70.1.55
Atkins, C A, et al. "De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.)." Plant physiology vol. 70,1 (1982): 55-60. doi: https://doi.org/10.1104/pp.70.1.55
Atkins CA, Ritchie A, Rowe PB, McCairns E, Sauer D. De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.). Plant Physiol. 1982 Jul;70(1):55-60. doi: 10.1104/pp.70.1.55. PMID: 16662479; PMCID: PMC1067085.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1982 Jul;70(1):55-60. doi: 10.1104/pp.70.1.55.

De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.).

Plant physiology

C A Atkins, A Ritchie, P B Rowe, E McCairns, D Sauer

Affiliations

  1. Department of Botany, University of Western Australia, Nedlands 6009, Australia.

PMID: 16662479 PMCID: PMC1067085 DOI: 10.1104/pp.70.1.55

Abstract

Partially purified, cell-free extracts from nodules of cowpea (Vigna unguiculata L. Walp. cv. Caloona) and soybean (Glycine max L. Merr. cv. Bragg) showed high rates of de novo purine nucleotide and purine base synthesis. Activity increased with rates of nitrogen fixation and ureide export during development of cowpea plants; maximum rates (equivalent to 1.2 micromoles N(2) per hour per gram fresh nodule) being similar to those of maximum nitrogen fixation (1-2 micromoles N(2) per hour per gram fresh nodule). Extracts from actively fixing nodules of a symbiosis not producing ureides, Lupinus albus L. cv. Ultra, showed rates of de novo purine synthesis 0.1% to 0.5% those of cowpea and soybean. Most (70-90%) of the activity was associated with the particulate components of the nodule, but up to 50% was released from this fraction by osmotic shock. The accumulated end products with particulate fractions were inosine monophosphate and aminoimidazole carboxamide ribonucleotide. Further metabolism to purine bases and ureides was restricted to the soluble fraction of the nodule extract. High rates of inosine monophosphate synthesis were supported by glutamine as amide donor, lower rates (10-20%) by ammonia, and negligible rates with asparagine as substrate.

References

  1. Plant Physiol. 1980 Jun;65(6):1203-6 - PubMed
  2. J Biol Chem. 1973 Mar 25;248(6):1970-5 - PubMed
  3. Biochem Biophys Res Commun. 1962 Sep 25;9:7-10 - PubMed
  4. J Biol Chem. 1978 Nov 10;253(21):7711-21 - PubMed
  5. Plant Physiol. 1980 May;65(5):961-5 - PubMed
  6. Biochemistry. 1977 Mar 22;16(6):1093-102 - PubMed
  7. Plant Physiol. 1977 Nov;60(5):800-2 - PubMed
  8. Plant Physiol. 1975 Dec;56(6):807-12 - PubMed
  9. Plant Physiol. 1981 Jul;68(1):65-9 - PubMed
  10. Plant Physiol. 1980 Oct;66(4):735-9 - PubMed
  11. Plant Physiol. 1978 Oct;62(4):495-8 - PubMed
  12. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  13. Plant Physiol. 1977 Apr;59(4):741-4 - PubMed

Publication Types