Display options
Cite
Sparace SA, Mudd JB. Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule. Plant Physiol. 1982;70(5):1260-4doi: 10.1104/pp.70.5.1260.
Sparace, S. A., & Mudd, J. B. (1982). Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule. Plant physiology, 70(5), 1260-4. https://doi.org/10.1104/pp.70.5.1260
Sparace, S A, and Mudd, J B. "Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule." Plant physiology vol. 70,5 (1982): 1260-4. doi: https://doi.org/10.1104/pp.70.5.1260
Sparace SA, Mudd JB. Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule. Plant Physiol. 1982 Nov;70(5):1260-4. doi: 10.1104/pp.70.5.1260. PMID: 16662664; PMCID: PMC1065872.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1982 Nov;70(5):1260-4. doi: 10.1104/pp.70.5.1260.

Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule.

Plant physiology

S A Sparace, J B Mudd

Affiliations

  1. Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92521.

PMID: 16662664 PMCID: PMC1065872 DOI: 10.1104/pp.70.5.1260

Abstract

Intact chloroplasts from spinach (Spinacia oleracea L., hybrid 424) readily incorporate [(14)C]glycerol-3-phosphate and [(14)C]acetate into diacylglycerol, monoacylglycerol, diacylglycrol, free fatty acids (only when acetate is the precursor), phosphatidic acid, phosphatidylcholine, and most notably phosphatidylglycerol. The fraction of phosphatidylglycerol synthesized is greatly increased by the presence of manganese chloride in the reaction mixture. Glycerol-3-phosphate-labeled phosphatidylglycerol is equally labeled in the two glycerol moieties of the molecule. Acetate-labeled phosphatidylglycerol is equally labeled in both acyl groups. Position one contains primarily oleate, linoleate and small amounts of palmitate. Position two contains primarily palmitate. No radioactive trans-Delta(3)-hexadecenoate was detected. The labeling patterns indicate that the radioactive phosphatidylglycerol is the product of de novo chloroplast lipid biosynthesis and furthermore, phosphatidylglycerol may be a substrate for fatty acid desaturation.

References

  1. Biochem J. 1979 Dec 15;184(3):571-4 - PubMed
  2. Plant Physiol. 1974 Aug;54(2):164-8 - PubMed
  3. Biochem Biophys Res Commun. 1965 Dec 9;21(5):473-9 - PubMed
  4. Biochim Biophys Acta. 1972 Apr 18;260(4):558-70 - PubMed
  5. Can J Biochem. 1969 Mar;47(3):257-63 - PubMed
  6. Hoppe Seylers Z Physiol Chem. 1973 Sep;354(9):1115-23 - PubMed
  7. Biochem J. 1976 Sep 15;158(3):593-601 - PubMed
  8. Biochem J. 1969 May;112(5):787-94 - PubMed
  9. Biochem J. 1979 Jun 1;179(3):649-56 - PubMed
  10. Biochim Biophys Acta. 1965 Jul 7;106(1):78-92 - PubMed
  11. Plant Physiol. 1981 Dec;68(6):1257-63 - PubMed
  12. Arch Biochem Biophys. 1981 Jul;209(2):584-91 - PubMed
  13. Arch Biochem Biophys. 1979 Oct 1;197(1):322-32 - PubMed
  14. FEBS Lett. 1970 Nov 18;11(2):121-124 - PubMed
  15. FEBS Lett. 1979 Jun 1;102(1):147-50 - PubMed
  16. Can J Biochem. 1966 Apr;44(4):459-67 - PubMed
  17. Plant Physiol. 1970 Jun;45(6):719-22 - PubMed
  18. Eur J Biochem. 1978 Oct;90(2):223-9 - PubMed
  19. Plant Physiol. 1981 Sep;68(3):653-7 - PubMed

Publication Types