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Plant Physiol. 1983 Aug;72(4):1136-8. doi: 10.1104/pp.72.4.1136.

Distribution of calmodulin-stimulated ca transport into membrane vesicles from green spinach leaves.

Plant physiology

V Stosic, C Penel, D Marme, H Greppin

Affiliations

  1. Laboratoire de Physiologie végétale, Université de Genève, 1211 Genève 4, Switzerland.

PMID: 16663135 PMCID: PMC1066389 DOI: 10.1104/pp.72.4.1136

Abstract

A microsomal fraction isolated from green spinach leaves exhibited a Mg(2+) and ATP-dependent (45)Ca(2+) uptake. Addition of 10 micromolar carbonyl cyanide m-chlorophenylhydrazine had no effect. The cationophore A23187 (10 micromolar) induced the release of (45)Ca(2+) accumulated by membrane vesicles. Membranes prepared from lower epidermis showed the highest Ca(2+) accumulation activity. Microsomal fractions from petiole, lamina, and midrib were less active. The stimulation by bovine brain calmodulin was about 30% for the lower epidermis, 23.5% for midrib, and below 20% for petiole and lamina.

References

  1. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7311-4 - PubMed
  2. Arch Biochem Biophys. 1976 Apr;173(2):720-31 - PubMed
  3. Biochim Biophys Acta. 1969;193(2):338-49 - PubMed
  4. Biochem Biophys Res Commun. 1979 Oct 12;90(3):1048-56 - PubMed
  5. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1232-6 - PubMed
  6. Anal Biochem. 1978 May;86(1):142-6 - PubMed
  7. Plant Physiol. 1982 Jan;69(1):103-6 - PubMed
  8. Biochim Biophys Acta. 1977 Sep 14;461(3):500-12 - PubMed

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