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Jourdan PS, Weiler EW, Mansell RL. Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin. Plant Physiol. 1985;77(4):896-902doi: 10.1104/pp.77.4.896.
Jourdan, P. S., Weiler, E. W., & Mansell, R. L. (1985). Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin. Plant physiology, 77(4), 896-902. https://doi.org/10.1104/pp.77.4.896
Jourdan, P S, et al. "Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin." Plant physiology vol. 77,4 (1985): 896-902. doi: https://doi.org/10.1104/pp.77.4.896
Jourdan PS, Weiler EW, Mansell RL. Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin. Plant Physiol. 1985 Apr;77(4):896-902. doi: 10.1104/pp.77.4.896. PMID: 16664158; PMCID: PMC1064627.
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Plant Physiol. 1985 Apr;77(4):896-902. doi: 10.1104/pp.77.4.896.

Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin.

Plant physiology

P S Jourdan, E W Weiler, R L Mansell

Affiliations

  1. Department of Biology, University of South Florida, Tampa, Florida 33620.

PMID: 16664158 PMCID: PMC1064627 DOI: 10.1104/pp.77.4.896

Abstract

The preparation of a tritiated radiotracer that was used in the radioimmunoassay of naringin (naringenin-7-O-alpha-rhamnosyl- (1-2)-beta-d-glucopyranoside) and which was synthesized by reduction of the carbonyl group of the flavanone is reported. The resulting assay has a detection limit of 0.5 picomole per 0.1 milliliter, is specific for the 7-neohesperidoside substitution on flavanones, and can measure naringin in crude extracts of plant tissues. This radioimmunoassay is compared with three other naringin immunoassays which use antibodies raised against two different haptens and different tracers labeled with (125)I or (3)H. The applicability of the methods to the quantification of naringin and other flavanone neohesperidosides in citrus tissue is discussed.

References

  1. Anal Biochem. 1984 Apr;138(1):19-24 - PubMed
  2. Planta Med. 1982 Feb;44(2):82-6 - PubMed
  3. Methods Enzymol. 1980;70(A):201-9 - PubMed
  4. J Immunoassay. 1983;4(1):83-98 - PubMed
  5. Science. 1978 Jun 16;200(4347):1236-45 - PubMed
  6. Methods Enzymol. 1980;70(A):104-42 - PubMed
  7. Methods Enzymol. 1980;70(A):85-104 - PubMed
  8. Anal Biochem. 1965 Sep;12(3):472-82 - PubMed
  9. Nature. 1959 Nov 21;184 (Suppl 21):1648-9 - PubMed

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