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Bottini R, de Bottini G, Koshioka M, et al. Identification of Gibberellins A(1), A(5), A(29), and A(32) from Immature Seeds of Apricot (Prunus armeniaca L.). Plant Physiol. 1985;78(2):417-9doi: 10.1104/pp.78.2.417.
Bottini, R., de Bottini, G., Koshioka, M., Pharis, R. P., & Coombe, B. G. (1985). Identification of Gibberellins A(1), A(5), A(29), and A(32) from Immature Seeds of Apricot (Prunus armeniaca L.). Plant physiology, 78(2), 417-9. https://doi.org/10.1104/pp.78.2.417
Bottini, R, et al. "Identification of Gibberellins A(1), A(5), A(29), and A(32) from Immature Seeds of Apricot (Prunus armeniaca L.)." Plant physiology vol. 78,2 (1985): 417-9. doi: https://doi.org/10.1104/pp.78.2.417
Bottini R, de Bottini G, Koshioka M, Pharis RP, Coombe BG. Identification of Gibberellins A(1), A(5), A(29), and A(32) from Immature Seeds of Apricot (Prunus armeniaca L.). Plant Physiol. 1985 Jun;78(2):417-9. doi: 10.1104/pp.78.2.417. PMID: 16664256; PMCID: PMC1064746.
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Plant Physiol. 1985 Jun;78(2):417-9. doi: 10.1104/pp.78.2.417.

Identification of Gibberellins A(1), A(5), A(29), and A(32) from Immature Seeds of Apricot (Prunus armeniaca L.).

Plant physiology

R Bottini, G de Bottini, M Koshioka, R P Pharis, B G Coombe

Affiliations

  1. Plant Physiology Research Group, Department of Biology, The University of Calgary, Calgary, Alberta, Canada T2N 1N4.

PMID: 16664256 PMCID: PMC1064746 DOI: 10.1104/pp.78.2.417

Abstract

Gibberellins (GAs) A(1), A(5), and A(29) were identified, and also GA(32) was confirmed, as endogenous GAs of immature seeds (3-4 weeks after anthesis, 0.25-0.5 gram fresh weight) of apricot (Prunus armeniaca L.) based on capillary gas chromatography (GC), retention time (Rt), and selected ion monitoring (SIM), in comparison with authentic standards. Fractions subjected to GC-SIM were purified and separated using sequential solvent partitioning --> paper chromatography --> reverse phase C(18) high performance liquid chromatography (HPLC) --> bioassay on dwarf rice cv Tan-ginbozu. Two other peaks of free GA-like bioactivity (microdrop and immersion dwarf rice assays) were eluted from C(18) HPLC at Rts where GA(4/7) and GA(8) (or other GAs with similar structures) would elute. Also, three unidentified GA glucoside-like compounds (based on bioactivity on the immersion assay, and no bioactivity on the microdrop assay) were noted. There were very high amounts of GA(32) (112 ng of GA(3) equivalents per gram fresh weight), and minor amounts (0.5 ng of GA(3) equivalents) for each of GA(1) and GA(5), respectively, based on the microdrop assay.

References

  1. Plant Physiol. 1979 Jan;63(1):129-32 - PubMed
  2. Science. 1971 May 21;172(3985):856-7 - PubMed

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