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Singh NK, Handa AK, Hasegawa PM, et al. Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. Plant Physiol. 1985;79(1):126-37doi: 10.1104/pp.79.1.126.
Singh, N. K., Handa, A. K., Hasegawa, P. M., & Bressan, R. A. (1985). Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. Plant physiology, 79(1), 126-37. https://doi.org/10.1104/pp.79.1.126
Singh, N K, et al. "Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl." Plant physiology vol. 79,1 (1985): 126-37. doi: https://doi.org/10.1104/pp.79.1.126
Singh NK, Handa AK, Hasegawa PM, Bressan RA. Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. Plant Physiol. 1985 Sep;79(1):126-37. doi: 10.1104/pp.79.1.126. PMID: 16664357; PMCID: PMC1074839.
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Plant Physiol. 1985 Sep;79(1):126-37. doi: 10.1104/pp.79.1.126.

Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl.

Plant physiology

N K Singh, A K Handa, P M Hasegawa, R A Bressan

Affiliations

  1. Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.

PMID: 16664357 PMCID: PMC1074839 DOI: 10.1104/pp.79.1.126

Abstract

Cultured tobacco cells (Nicotiana tabacum L. cv Wisconsin 38) adapted to grow in medium containing high levels of NaCl or polyethylene glycol (PEG) produce several new or enhanced polypeptide bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The intensities of some of the polypeptide bands (molecular weights of 58, 37, 35.5, 34, 26, 21, 19.5, and 18 kilodaltons) increase with increasing levels of NaCl adaptation, while the intensities of other polypeptide bands (54, 52, 17.5, and 16.5 kilodaltons) are reduced. Enhanced levels of 43- and 26-kilodalton polypeptides are present in both NaCl and PEG-induced water stress adapted cells but are not detectable in unadapted cells. In addition, PEG adapted cells have enhanced levels of 29-, 17.5-, 16.5-, and 11-kilodalton polypeptides and reduced levels of 58-, 54-, 52-, 37-, 35.5-, 34-, 21-, 19.5-, and 18-kilodalton polypeptide bands.Synthesis of 26-kilodalton polypeptide(s) occurs at two different periods during culture growth of NaCl adapted cells. Unadapted cells also incorporate (35)S into a 26-kilodalton polypeptide during the later stage of culture growth beginning at midlog phase. The 26-kilodalton polypeptides from adapted and unadapted cells have similar partial proteolysis peptide maps and are immunologically cross-reactive. During adaptation to NaCl, unadapted cells synthesize and accumulate a major 26-kilodalton polypeptide, and the beginning of synthesis corresponds to the period of osmotic adjustment and culture growth. From our results, we suggest an involvement of the 26-kilodalton polypeptide in the adaptation of cultured tobacco cells to NaCl and water stress.

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