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Kombrink E, Hahlbrock K. Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions. Plant Physiol. 1986;81(1):216-21doi: 10.1104/pp.81.1.216.
Kombrink, E., & Hahlbrock, K. (1986). Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions. Plant physiology, 81(1), 216-21. https://doi.org/10.1104/pp.81.1.216
Kombrink, E, and Hahlbrock, K. "Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions." Plant physiology vol. 81,1 (1986): 216-21. doi: https://doi.org/10.1104/pp.81.1.216
Kombrink E, Hahlbrock K. Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions. Plant Physiol. 1986 May;81(1):216-21. doi: 10.1104/pp.81.1.216. PMID: 16664778; PMCID: PMC1075309.
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Plant Physiol. 1986 May;81(1):216-21. doi: 10.1104/pp.81.1.216.

Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions.

Plant physiology

E Kombrink, K Hahlbrock

Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, 5000 Köln 30, Federal Republic of Germany.

PMID: 16664778 PMCID: PMC1075309 DOI: 10.1104/pp.81.1.216

Abstract

Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). The activities of two hydrolytic enzymes, chitinase and 1,3-beta-glucanase, also increased strongly in elicitor-treated cells, whereas the activities of three enzymes participating in primary metabolism were affected differently by the elicitor treatment. Glucose-6-phosphate dehydrogenase increased, phosphofructokinase remained almost constant, and pyrophosphate:fructose-6-phosphate phosphotransferase declined sharply in activity. Different amounts of cell-wall preparations from various phytopathogenic fungi were required for maximum elicitor activity. While three oomycetes (Phytophthora spp.) yielded the most active elicitors studied (maximum coumarin accumulation at concentrations of about 10 microgram per milliliter), cell-wall preparations from an ascomycete and three deuteromycetes gave comparable results only at 10 to 100 times higher concentrations. Optimal induction of PAL, 4CL, and chitinase with Phytophthora elicitor required only about 1 microgram per milliliter, whereas 1,3-beta-glucanase induction showed a dose dependence similar to that observed for coumarins. The elicitor concentration had pronounced effects not only on the extent, but also on the timing of all induced reactions.

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