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Piazza GJ, Saggese EJ, Spletzer KM. Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds. Plant Physiol. 1987;83(1):177-80doi: 10.1104/pp.83.1.177.
Piazza, G. J., Saggese, E. J., & Spletzer, K. M. (1987). Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds. Plant physiology, 83(1), 177-80. https://doi.org/10.1104/pp.83.1.177
Piazza, G J, et al. "Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds." Plant physiology vol. 83,1 (1987): 177-80. doi: https://doi.org/10.1104/pp.83.1.177
Piazza GJ, Saggese EJ, Spletzer KM. Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds. Plant Physiol. 1987 Jan;83(1):177-80. doi: 10.1104/pp.83.1.177. PMID: 16665197; PMCID: PMC1056319.
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Plant Physiol. 1987 Jan;83(1):177-80. doi: 10.1104/pp.83.1.177.

Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds.

Plant physiology

G J Piazza, E J Saggese, K M Spletzer

Affiliations

  1. United States Department of Agriculture, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.

PMID: 16665197 PMCID: PMC1056319 DOI: 10.1104/pp.83.1.177

Abstract

Recognized calmodulin antagonists and chlorinated phenoxyalkylamines were tested as inhibitors of mevalonate incorporation into triterpenols and their fatty acid esters in a centrifuged pellet from the latex of Euphorbia lathyris. The calmodulin antagonists, chlorpromazine (II), fluphenzine, and trifluoperazine were good inhibitors; I(50) values for II and trifluoperazine were 150 and 55 micromolar, respectively. Inhibition by the phenoxyalkylamines increased with increasing chlorine substitution, and I(50) for 2-(pentachlorophenoxy)ethyl N,N-diethylamine (IX) was 35 micromolar. The calmodulin-stimulated phosphodiesterase catalyzed hydrolysis of cAMP was used as an assay to quantitate the calmodulin antagonism of the tested compounds. Compounds II and IX were calmodulin antagonists over a concentration range similar to their effective range in the biosynthesis of triterpenes. The antagonism of the chlorinated phenoxy compounds increased in parallel to their inhibitory effect upon triterpene biosynthesis.

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