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Stinson JR, Eisenberg AJ, Willing RP, et al. Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol. 1987;83(2):442-7doi: 10.1104/pp.83.2.442.
Stinson, J. R., Eisenberg, A. J., Willing, R. P., Pe, M. E., Hanson, D. D., & Mascarenhas, J. P. (1987). Genes expressed in the male gametophyte of flowering plants and their isolation. Plant physiology, 83(2), 442-7. https://doi.org/10.1104/pp.83.2.442
Stinson, J R, et al. "Genes expressed in the male gametophyte of flowering plants and their isolation." Plant physiology vol. 83,2 (1987): 442-7. doi: https://doi.org/10.1104/pp.83.2.442
Stinson JR, Eisenberg AJ, Willing RP, Pe ME, Hanson DD, Mascarenhas JP. Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol. 1987 Feb;83(2):442-7. doi: 10.1104/pp.83.2.442. PMID: 16665265; PMCID: PMC1056377.
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Plant Physiol. 1987 Feb;83(2):442-7. doi: 10.1104/pp.83.2.442.

Genes expressed in the male gametophyte of flowering plants and their isolation.

Plant physiology

J R Stinson, A J Eisenberg, R P Willing, M E Pe, D D Hanson, J P Mascarenhas

Affiliations

  1. Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222.

PMID: 16665265 PMCID: PMC1056377 DOI: 10.1104/pp.83.2.442

Abstract

Recombinant cDNA libraries to poly(A)RNA isolated from mature pollen of Zea mays and Tradescantia paludosa have been constructed. Northern blot analyses indicate that several of the clones are unique to pollen and are not expressed in vegetative tissues. The majority, however, are expressed both in pollen and vegetative tissues. Southern hybridizations show that the pollen specific sequences in corn are present in one or a very few copies in the genome. By using several of the clones as probes, it was found that there are at least two different groups of mRNAs with respect to their synthesis. The mRNAs of the first group represented by the pollen specific clones are synthesized after microspore mitosis and increase in concentration up to maturity. The second group, exemplified by actin mRNA, begins to accumulate soon after meiosis, reaches its maximum by late pollen interphase, and decreases thereafter. Although the actin mRNA and the pollen specific mRNAs studied show very different patterns of initiation of synthesis and accumulation during pollen development, the rates of decline of these mRNAs during the first 60 minutes of germination and pollen tube growth in Tradescantia are similar and reflect the previously observed declines in rates of protein synthesis during this period.

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