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Pearce D, Pharis RP, Rajasekaran K, et al. Effects of Chilling and ABA on [H]Gibberellin A(4) Metabolism in Somatic Embryos of Grape (Vitis vinifera L. x V. rupestris Scheele). Plant Physiol. 1987;84(2):381-5doi: 10.1104/pp.84.2.381.
Pearce, D., Pharis, R. P., Rajasekaran, K., & Mullins, M. G. (1987). Effects of Chilling and ABA on [H]Gibberellin A(4) Metabolism in Somatic Embryos of Grape (Vitis vinifera L. x V. rupestris Scheele). Plant physiology, 84(2), 381-5. https://doi.org/10.1104/pp.84.2.381
Pearce, D, et al. "Effects of Chilling and ABA on [H]Gibberellin A(4) Metabolism in Somatic Embryos of Grape (Vitis vinifera L. x V. rupestris Scheele)." Plant physiology vol. 84,2 (1987): 381-5. doi: https://doi.org/10.1104/pp.84.2.381
Pearce D, Pharis RP, Rajasekaran K, Mullins MG. Effects of Chilling and ABA on [H]Gibberellin A(4) Metabolism in Somatic Embryos of Grape (Vitis vinifera L. x V. rupestris Scheele). Plant Physiol. 1987 Jun;84(2):381-5. doi: 10.1104/pp.84.2.381. PMID: 16665447; PMCID: PMC1056587.
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Plant Physiol. 1987 Jun;84(2):381-5. doi: 10.1104/pp.84.2.381.

Effects of Chilling and ABA on [H]Gibberellin A(4) Metabolism in Somatic Embryos of Grape (Vitis vinifera L. x V. rupestris Scheele).

Plant physiology

D Pearce, R P Pharis, K Rajasekaran, M G Mullins

Affiliations

  1. Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4.

PMID: 16665447 PMCID: PMC1056587 DOI: 10.1104/pp.84.2.381

Abstract

Previous work has indicated that changes in gibberellin (GA) metabolism may be involved in chilling-induced release from dormancy in somatic embryos of grape (Vitis vinifera L. x V. rupestris Scheele). We have chilled somatic embryos of grape for 2, 4, or 8 weeks, then incubated them with [(3)H]GA(4) (of high specific activity, 4.81 x 10(10) becquerel per millimole) for 48 hours at 26 degrees C. Chilling had little effect on the total amount of free [(3)H]GA-like metabolites formed during incubation at 26 degrees C, but did change the relative proportions of individual metabolites. The amount of highly water-soluble [(3)H] metabolites formed at 26 degrees C decreased in embryos chilled for 4 or 8 weeks. The concentration of endogenous GA precursors (e.g., GA(12) aldehyde-, kaurene-, and kaurenoic acid-like substances) increased in embryos chilled for 4 or 8 weeks. Treatment with abscisic acid (ABA) (known to inhibit germination in grape embryos) concurrent with [(3)H]GA(4) treatment at 26 degrees C, reduced the uptake of [(3)H] GA(4) but had little effect on the qualitative spectrum of metabolites. However, in the embryos chilled for 8 weeks and then treated with ABA for 48 hours at 26 degrees C, there was a higher concentration of GA precursors than in untreated control embryos. Chilled embryos thus have an enhanced potential for an increase in free GAs through synthesis from increased amounts of GA precursors, or through a reduced ability to form highly water-soluble GA metabolites (i.e., GA conjugates or polyhydroxylated free GAs).

References

  1. Plant Physiol. 1983 Oct;73(2):398-406 - PubMed
  2. Plant Physiol. 1983 Nov;73(3):803-8 - PubMed

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