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Oleski N, Mahdavi P, Bennett AB. Transport Properties of the Tomato Fruit Tonoplast : II. Citrate Transport. Plant Physiol. 1987;84(4):997-1000doi: 10.1104/pp.84.4.997.
Oleski, N., Mahdavi, P., & Bennett, A. B. (1987). Transport Properties of the Tomato Fruit Tonoplast : II. Citrate Transport. Plant physiology, 84(4), 997-1000. https://doi.org/10.1104/pp.84.4.997
Oleski, N, et al. "Transport Properties of the Tomato Fruit Tonoplast : II. Citrate Transport." Plant physiology vol. 84,4 (1987): 997-1000. doi: https://doi.org/10.1104/pp.84.4.997
Oleski N, Mahdavi P, Bennett AB. Transport Properties of the Tomato Fruit Tonoplast : II. Citrate Transport. Plant Physiol. 1987 Aug;84(4):997-1000. doi: 10.1104/pp.84.4.997. PMID: 16665636; PMCID: PMC1056716.
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Plant Physiol. 1987 Aug;84(4):997-1000. doi: 10.1104/pp.84.4.997.

Transport Properties of the Tomato Fruit Tonoplast : II. Citrate Transport.

Plant physiology

N Oleski, P Mahdavi, A B Bennett

Affiliations

  1. Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616.

PMID: 16665636 PMCID: PMC1056716 DOI: 10.1104/pp.84.4.997

Abstract

Citrate transport across the membrane of tomato fruit tonoplast vesicles was investigated. In the tonoplast vesicles, [(14)C]methylamine uptake was stimulated 10-fold by MgATP and strongly inhibited by NO(3) (-). Under identical experimental conditions, [(14)C]citrate uptake was inhibited by 5 millimolar free Mg(2+), and this inhibition was reversed in the presence of ATP, presumably by ATP chelation of free Mg(2+). No evidence was obtained in support of energy-linked ATP stimulation of citrate uptake. Citrate uptake showed saturation kinetics, and was inhibited by 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid and by other organic acids. The pH-dependence of uptake suggested that citrate(3-) was the transported species. Our results indicate that citrate transport across the tomato fruit tonoplast occurs by facilitated diffusion of citrate(3-). The carrier shares some features in common with anion channels in that it is relatively nonspecific for organic acids and is inhibitable by 4,4'-diisothyocyano-2,2'-stilbenedisulfonic acid.

References

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