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Granerus M, Bierke P, Zumkeller W, et al. Insulin-like growth factor II prevents apoptosis in a human teratoma derived cell line. Clin Mol Pathol. 1995;48(3):M153-7doi: 10.1136/mp.48.3.m153.
Granerus, M., Bierke, P., Zumkeller, W., Smith, J., Engström, W., & Schofield, P. N. (1995). Insulin-like growth factor II prevents apoptosis in a human teratoma derived cell line. Clinical molecular pathology, 48(3), M153-7. https://doi.org/10.1136/mp.48.3.m153
Granerus, M, et al. "Insulin-like growth factor II prevents apoptosis in a human teratoma derived cell line." Clinical molecular pathology vol. 48,3 (1995): M153-7. doi: https://doi.org/10.1136/mp.48.3.m153
Granerus M, Bierke P, Zumkeller W, Smith J, Engström W, Schofield PN. Insulin-like growth factor II prevents apoptosis in a human teratoma derived cell line. Clin Mol Pathol. 1995 Jun;48(3):M153-7. doi: 10.1136/mp.48.3.m153. PMID: 16695997; PMCID: PMC407949.
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Clin Mol Pathol. 1995 Jun;48(3):M153-7. doi: 10.1136/mp.48.3.m153.

Insulin-like growth factor II prevents apoptosis in a human teratoma derived cell line.

Clinical molecular pathology

M Granerus, P Bierke, W Zumkeller, J Smith, W Engström, P N Schofield

Affiliations

  1. Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, S-75007 Uppsala, Sweden.

PMID: 16695997 PMCID: PMC407949 DOI: 10.1136/mp.48.3.m153

Abstract

Aim-To study how insulin-like growth factor II (IGF-II) affects the behaviour of human teratoma cells.Methods-The human pluripotential teratoma cell line Tera 2 was cultured under serum-free conditions in the presence or absence of IGF-II. Effects on cell proliferation and apoptosis as well as on the expression of the proto-oncogene c-myc were studied.Results-In this study we show that Tera 2 cells deprived of serum undergo programmed cell death (apoptosis). The onset of nuclear fragmentation was observed 12 hours after serum withdrawal. The morphological changes of the Tera 2 cell nuclei were confirmed by the occurrence of a nucleosome ladder. However, the constitutive expression of the proto-oncogene c-myc was not decreased in parallel with initiation of apoptosis. The apoptotic response to serum withdrawal could be counteracted by simultaneous addition of IGF-II. In addition it was found that human testicular tumours (seminoma and embryonal carcinoma) contain raised levels of insulin-like growth factors.Conclusions-The precise roles of IGF-I and IGF-II have been unclear, and there is overwhelming evidence against these factors as primarily transforming agents. The finding that IGF-II apparently counteracts apoptosis in vitro may well explain its effects on tumours in vivo.

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