Display options
Share it on

Plant Physiol. 1988 Aug;87(4):859-66. doi: 10.1104/pp.87.4.859.

Determination of the Processing Sites of an Arabidopsis 2S Albumin and Characterization of the Complete Gene Family.

Plant physiology

E Krebbers, L Herdies, A De Clercq, J Seurinck, J Leemans, J Van Damme, M Segura, G Gheysen, M Van Montagu, J Vandekerckhove

Affiliations

  1. Plant Genetic Systems, J. Plateaustraat 22, B-9000 Gent, Belgium.

PMID: 16666238 PMCID: PMC1054859 DOI: 10.1104/pp.87.4.859

Abstract

The most abundant isoform of the 2S albumin present in seeds of Arabidopsis thaliana has been sequenced and the corresponding gene isolated. Examination of the protein and DNA sequences allows the determination of the exact proteolytic cleavage sites during posttranslational processing. Like other 2S albumins, that of Arabidopsis is made as a prepropeptide. After removal of the signal peptide, the propeptide is cleaved at four other points, giving two subunits linked by a disulfide bridge(s). Comparison of these cleavage sites with those of 2S albumins of Brassica napus and Bertholletia excelsa suggests that while individual cleavage sites between species are conserved, the four processing sites within a species are not similar, suggesting that up to four different proteases may be involved in processing 2S albumins. The Arabidopsis 2S albumin gene was used to isolate the entire gene family. There are four genes, tightly linked in a tandem array. None of the genes contains an intron. Comparison of the predicted protein sequences shows that only one of the genes can encode the isoform determined by protein analysis to be the most abundant, and therefore this gene is certain to be expressed. It is possible that some or all of the other three genes are also active.

References

  1. Methods Enzymol. 1983;91:486-93 - PubMed
  2. Gene. 1985;33(1):103-19 - PubMed
  3. Methods Enzymol. 1980;65(1):499-560 - PubMed
  4. Plant Physiol. 1984 Jul;75(3):651-7 - PubMed
  5. Biochim Biophys Acta. 1972 Aug 31;278(1):175-83 - PubMed
  6. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
  7. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123-7 - PubMed
  8. J Mol Biol. 1986 Jan 20;187(2):169-83 - PubMed
  9. Gene. 1983 Dec;26(2-3):171-9 - PubMed
  10. Eur J Biochem. 1986 Sep 15;159(3):597-604 - PubMed
  11. J Mol Biol. 1983 Jun 25;167(2):391-409 - PubMed
  12. Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 - PubMed
  13. Proc Natl Acad Sci U S A. 1975 Feb;72(2):619-22 - PubMed
  14. Anal Biochem. 1984 May 1;138(2):267-84 - PubMed
  15. J Cell Biol. 1982 May;93(2):306-13 - PubMed
  16. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1931-5 - PubMed
  17. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  18. Nucleic Acids Res. 1986 Jan 10;14(1):363-74 - PubMed
  19. J Mol Appl Genet. 1983;2(3):273-83 - PubMed
  20. J Biol Chem. 1987 Sep 5;262(25):12202-8 - PubMed
  21. J Biol Chem. 1982 Dec 25;257(24):14753-9 - PubMed
  22. Cell. 1982 Jun;29(2):651-60 - PubMed
  23. J Biol Chem. 1986 Nov 5;261(31):14576-81 - PubMed
  24. Gene. 1984 Jun;28(3):351-9 - PubMed

Publication Types