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Zumkeller W, Mahmood A, Dellow R, et al. IGF-II dependent autocrine growth in cell lines derived from renal tumours of childhood. Clin Mol Pathol. 1995;48(6):M333-41doi: 10.1136/mp.48.6.m333.
Zumkeller, W., Mahmood, A., Dellow, R., & Schofield, P. N. (1995). IGF-II dependent autocrine growth in cell lines derived from renal tumours of childhood. Clinical molecular pathology, 48(6), M333-41. https://doi.org/10.1136/mp.48.6.m333
Zumkeller, W, et al. "IGF-II dependent autocrine growth in cell lines derived from renal tumours of childhood." Clinical molecular pathology vol. 48,6 (1995): M333-41. doi: https://doi.org/10.1136/mp.48.6.m333
Zumkeller W, Mahmood A, Dellow R, Schofield PN. IGF-II dependent autocrine growth in cell lines derived from renal tumours of childhood. Clin Mol Pathol. 1995 Dec;48(6):M333-41. doi: 10.1136/mp.48.6.m333. PMID: 16696034; PMCID: PMC408001.
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Clin Mol Pathol. 1995 Dec;48(6):M333-41. doi: 10.1136/mp.48.6.m333.

IGF-II dependent autocrine growth in cell lines derived from renal tumours of childhood.

Clinical molecular pathology

W Zumkeller, A Mahmood, R Dellow, P N Schofield

Affiliations

  1. Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, United Kingdom.

PMID: 16696034 PMCID: PMC408001 DOI: 10.1136/mp.48.6.m333

Abstract

Aims-To determine the role of insulin-like growth factors (IGF) in the proliferation of tumour cells, by studying the mitogenic response to IGFs of three cell lines of differing phenotype established from both malignant rhabdoid and Wilms tumour, representing a range of cell types (GOS 4, G401, and T3/73).Methods-Production of IGF-II and IGF-I was measured by radioimmunoassay, and the presence of IGF binding protein complexes was observed by gel exclusion chromatography. Following growth analyses in serum-free media to ascertain the dependence of the cell lines on exogenous IGFs, the generation of autocrine growth was measured by a density dependence assay of proliferation in culture. Receptors were measured by radioligand cross linking and autocrine growth through these receptors assayed by the use of blocking antibodies.Results-While GOS 4 and G401 were able to proliferate in serum-free medium over a period of 5 d, T3/73 showed an absolute dependence on IGFs added daily at 1-10 ng/ml. Plating at clonal density showed that cell growth was directly density dependent in serum-free medium. The serum independent proliferation of G401 and GOS 4 was blocked by the addition of an antibody to the type 1 IGF receptor (alpha-IR3) suggesting that the effects of autocrine factors are mediated through type 1 IGF receptors. S1 nuclease protection analysis indicated that all three cell lines produced significant amounts of mRNA derived mainly from the P3 IGF-II promoter, but transcripts for IGF-I were undetectable. Radioimmunoassay of IGFs from conditioned media showed that all the lines made assayable IGF-II (8.6, 8.4, and 6.1 ng/ml/24 h/10(6) cells for GOS 4, G401, and T3/73 respectively). The presence of species consistent with both type 1 and type II IGF receptors was demonstrated using radioligand binding to cell membranes followed by cross linking.Conclusions-Autocrine IGF-II may contribute to the serum independence of GOS 4 and G401 cells, whereas T3/73 may depend on exogenous IGF-II for proliferation.

References

  1. Semin Diagn Pathol. 1994 May;11(2):126-35 - PubMed
  2. Proc Natl Acad Sci U S A. 1987 Feb;84(4):1104-6 - PubMed
  3. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5828-32 - PubMed
  4. J Biol Chem. 1983 May 25;258(10):6561-6 - PubMed
  5. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10365-9 - PubMed
  6. Am J Pathol. 1993 Feb;142(2):375-80 - PubMed
  7. Carcinogenesis. 1987 Jun;8(6):865-8 - PubMed
  8. Nat Genet. 1994 Jul;7(3):440-7 - PubMed
  9. Br J Cancer. 1992 Mar;65(3):311-20 - PubMed
  10. Nat Genet. 1993 Oct;5(2):143-50 - PubMed
  11. Pediatr Pathol. 1994 Jul-Aug;14(4):723-30 - PubMed
  12. Cancer Res. 1994 Jun 1;54(11):2934-42 - PubMed
  13. Mol Cell Biol. 1994 Jun;14(6):3604-12 - PubMed
  14. Hum Mol Genet. 1994 Jul;3(7):1117-21 - PubMed
  15. Nature. 1985 Sep 19-25;317(6034):258-60 - PubMed
  16. Pediatr Pathol. 1990;10(1-2):1-36 - PubMed
  17. Am J Pathol. 1987 Nov;129(2):353-63 - PubMed
  18. J Cell Sci. 1988 Jul;90 ( Pt 3):475-84 - PubMed
  19. Early Hum Dev. 1990 Jan;21(1):49-58 - PubMed
  20. Nature. 1993 Apr 22;362(6422):749-51 - PubMed
  21. J Mol Endocrinol. 1989 May;2(3):201-6 - PubMed
  22. J Cell Sci. 1987 Aug;88 ( Pt 1):57-64 - PubMed
  23. Lab Invest. 1993 Nov;69(5):603-15 - PubMed
  24. Am J Pathol. 1993 Dec;143(6):1535-42 - PubMed
  25. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903 - PubMed
  26. Cancer Res. 1992 Apr 1;52(7):1830-5 - PubMed
  27. Eur J Pediatr. 1989 Jun;148(7):620-3 - PubMed
  28. Nat Genet. 1993 May;4(1):94-7 - PubMed
  29. Science. 1992 Jul 31;257(5070):674-8 - PubMed
  30. Am J Pathol. 1988 Mar;130(3):431-5 - PubMed
  31. J Cell Physiol. 1991 May;147(2):248-55 - PubMed
  32. Nature. 1985 Sep 19-25;317(6034):260-2 - PubMed
  33. J Biol Chem. 1988 Aug 15;263(23):11486-92 - PubMed
  34. Int J Cancer. 1987 Oct 15;40(4):499-504 - PubMed
  35. FEBS Lett. 1987 Apr 20;214(2):259-64 - PubMed
  36. Growth Regul. 1992 Mar;2(1):1-9 - PubMed
  37. Anticancer Res. 1989 Sep-Oct;9(5):1417-26 - PubMed
  38. Clin Mol Pathol. 1995 Jun;48(3):M153-7 - PubMed
  39. Eur J Cancer. 1993;29A(14):1973-7 - PubMed
  40. Am J Pathol. 1989 Dec;135(6):961-6 - PubMed
  41. J Cell Biol. 1991 Jun;113(6):1447-53 - PubMed
  42. Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;62(4):207-20 - PubMed
  43. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
  44. Mol Endocrinol. 1989 Nov;3(11):1701-9 - PubMed
  45. J Clin Invest. 1989 Sep;84(3):829-39 - PubMed
  46. Nat Genet. 1994 Jul;7(3):433-9 - PubMed
  47. Virchows Arch A Pathol Anat Histopathol. 1982;398(1):101-8 - PubMed
  48. Eur J Pediatr. 1993 Feb;152(2):102-6 - PubMed
  49. Genes Chromosomes Cancer. 1994 Oct;11(2):97-105 - PubMed
  50. Development. 1987 Dec;101(4):793-803 - PubMed
  51. Br J Cancer. 1991 May;63(5):687-92 - PubMed
  52. Nature. 1994 Jun 2;369(6479):414-8 - PubMed
  53. Cell Death Differ. 1995 Oct;2(4):243-51 - PubMed
  54. Anticancer Res. 1994 Mar-Apr;14(2A):533-8 - PubMed
  55. Lab Invest. 1989 Nov;61(5):522-6 - PubMed
  56. Science. 1987 Apr 10;236(4798):175-80 - PubMed
  57. Mol Reprod Dev. 1994 Nov;39(3):249-58 - PubMed

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