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Southern SA, Herrington CS. Assessment of intra-tumoral karyotypic heterogeneity by interphase cytogenetics in paraffin wax sections. Clin Mol Pathol. 1996;49(5):M283-9doi: 10.1136/mp.49.5.m283.
Southern, S. A., & Herrington, C. S. (1996). Assessment of intra-tumoral karyotypic heterogeneity by interphase cytogenetics in paraffin wax sections. Clinical molecular pathology, 49(5), M283-9. https://doi.org/10.1136/mp.49.5.m283
Southern, S A, and Herrington, C S. "Assessment of intra-tumoral karyotypic heterogeneity by interphase cytogenetics in paraffin wax sections." Clinical molecular pathology vol. 49,5 (1996): M283-9. doi: https://doi.org/10.1136/mp.49.5.m283
Southern SA, Herrington CS. Assessment of intra-tumoral karyotypic heterogeneity by interphase cytogenetics in paraffin wax sections. Clin Mol Pathol. 1996 Oct;49(5):M283-9. doi: 10.1136/mp.49.5.m283. PMID: 16696090; PMCID: PMC408074.
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Clin Mol Pathol. 1996 Oct;49(5):M283-9. doi: 10.1136/mp.49.5.m283.

Assessment of intra-tumoral karyotypic heterogeneity by interphase cytogenetics in paraffin wax sections.

Clinical molecular pathology

S A Southern, C S Herrington

Affiliations

  1. University of Liverpool, Department of Pathology, Duncan Building, Royal Liverpool University Hospital, Liverpool L69 3BX.

PMID: 16696090 PMCID: PMC408074 DOI: 10.1136/mp.49.5.m283

Abstract

Aim-To analyse the effect of sectioning on the assessment of karyotypic heterogeneity by interphase cytogenetics in paraffin wax embedded normal squamous epithelium and to apply the principles derived to invasive cervical carcinoma.Methods-Normal male (n = 5) and female (n = 5) squamous epithelia were hybridised with peri-centromeric repeat probes specific for chromosomes X (DXZ1) and 17 (D17Z1) individually and in combination to assess the effect of sectioning on mono-, di-, tri-, and tetrasomic populations. Section thickness, interobserver variation and variation between different areas of the epithelium were evaluated. Invasive squamous carcinomas of the cervix (n = 5) were then hybridised with the DXZ1 probe and intratumoral heterogeneity was assessed by comparison of signal distributions obtained from different areas.Results-The optimum section thickness for the assessment of normal epithelium was 6 mum. Variation in the expected signal number in the range 1-4 did not introduce artefactual heterogeneity at this section thickness. The sensitivity of this approach for the detection of minor subpopulations was calculated to be 13-16%, 17-18% and 10-11% for mono-, tri- and tetrasomic populations, respectively. Karyotypic heterogeneity was detected in two of the five tumours and, in one case where the populations where clustered morphologically, a minor population representing 18% was identified.Conclusions-Interphase cytogenetic analysis of sections from paraffin wax embedded material can be used for the detection of minor subpopulations in tumours. This approach will be of particular value in the assessment of the relation between human papillomavirus infection and tumour karyotype and in the analysis of intraepithelial neoplasia.

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