Display options
Cite
Klein RR, Gamble PE, Mullet JE. Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant Physiol. 1988;88(4):1246-56doi: 10.1104/pp.88.4.1246.
Klein, R. R., Gamble, P. E., & Mullet, J. E. (1988). Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant physiology, 88(4), 1246-56. https://doi.org/10.1104/pp.88.4.1246
Klein, R R, et al. "Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement." Plant physiology vol. 88,4 (1988): 1246-56. doi: https://doi.org/10.1104/pp.88.4.1246
Klein RR, Gamble PE, Mullet JE. Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant Physiol. 1988 Dec;88(4):1246-56. doi: 10.1104/pp.88.4.1246. PMID: 16666451; PMCID: PMC1055749.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1988 Dec;88(4):1246-56. doi: 10.1104/pp.88.4.1246.

Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement.

Plant physiology

R R Klein, P E Gamble, J E Mullet

Affiliations

  1. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128.

PMID: 16666451 PMCID: PMC1055749 DOI: 10.1104/pp.88.4.1246

Abstract

The accumulation of radiolabeled plastid-encoded chlorophyll a-apoproteins is light dependent and is controlled at a posttranscriptional level. Illumination of dark-grown barley (Hordeum vulgare L.) with a brief pulse of red light induced the accumulation of radiolabeled chlorophyll a-apoproteins in subsequent protein synthesis assays. The induction of radiolabeled chlorophyll a-apoprotein accumulation was not affected by pretreatment of leaves with cycloheximide. Fluence response studies showed that a red light photoreceptor controls the accumulation of radiolabeled chlorophyll a-apoproteins with a threshold fluence of approximately 50 to 100 microeinsteins per square meter. While red light initiated chlorophyll a-apoprotein accumulation, this process was not reversed by a far red light treatment given immediately after the pulse of red light. The light pulse which initiated the accumulation of radiolabeled chlorophyll a-apoproteins also induced the rapid conversion of protochlorophyllide to chlorophyll a. A chlorophyll-deficient mutant, xan-f(10), which is blocked in chlorophyll biosynthesis prior to protochlorophyllide formation, failed to accumulate radiolabeled chlorophyll a-apoproteins in the light even though transcripts for these apoproteins were present. A second mutant, xan-j(64), which accumulates chlorophyllide in the light but only low levels of chlorophyll a, also showed reduced accumulation of radiolabeled chlorophyll a-apoproteins upon illumination. These results suggest that the light-induced conversion of protochlorophyllide to chlorophyll a is necessary for accumulation of the plastid-encoded chlorophyll a-apoproteins and one red light photoreceptor controlling this response is the protochlorophyllide holochrome.

References

  1. Biochem J. 1986 Jul 15;237(2):313-26 - PubMed
  2. J Cell Biol. 1988 Feb;106(2):289-301 - PubMed
  3. Plant Physiol. 1986 Aug;81(4):1033-8 - PubMed
  4. Eur J Biochem. 1986 Mar 3;155(2):331-8 - PubMed
  5. Plant Physiol. 1988 Mar;86(3):706-10 - PubMed
  6. Plant Physiol. 1988 Jan;86(1):299-305 - PubMed
  7. Plant Physiol. 1985 Oct;79(2):371-6 - PubMed
  8. Eur J Biochem. 1986 Feb 3;154(3):625-34 - PubMed
  9. Eur J Biochem. 1981 Nov;120(1):89-93 - PubMed
  10. Science. 1984 Dec 21;226(4681):1447-9 - PubMed
  11. J Biol Chem. 1987 Mar 25;262(9):4341-8 - PubMed
  12. Eur J Biochem. 1981 Nov;120(1):95-103 - PubMed
  13. Science. 1986 May 30;232(4754):1106-12 - PubMed
  14. J Biol Chem. 1986 Aug 25;261(24):11138-45 - PubMed
  15. Methods Enzymol. 1972;24:321-2 - PubMed

Publication Types