Display options
Cite
Fett WF, Dunn MF. Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts. Plant Physiol. 1989;89(1):5-9doi: 10.1104/pp.89.1.5.
Fett, W. F., & Dunn, M. F. (1989). Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts. Plant physiology, 89(1), 5-9. https://doi.org/10.1104/pp.89.1.5
Fett, W F, and Dunn, M F. "Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts." Plant physiology vol. 89,1 (1989): 5-9. doi: https://doi.org/10.1104/pp.89.1.5
Fett WF, Dunn MF. Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts. Plant Physiol. 1989 Jan;89(1):5-9. doi: 10.1104/pp.89.1.5. PMID: 16666545; PMCID: PMC1055789.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1989 Jan;89(1):5-9. doi: 10.1104/pp.89.1.5.

Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible Hosts.

Plant physiology

W F Fett, M F Dunn

Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.

PMID: 16666545 PMCID: PMC1055789 DOI: 10.1104/pp.89.1.5

Abstract

Bacterial exopolysaccharide (EPS) was extracted from infected leaves of several host plants inoculated with phytopathogenic strains of Pseudomonas syringae pathovars. Extraction was by a facilitated diffusion procedure or by collection of intercellular fluid using a centrifugation method. The extracted EPS was purified and characterized. All bacterial pathogens which induced watersoaked lesions on their host leaves, a characteristic of most members of this bacterial group, were found to produce alginic acid (a polymer consisting of varying ratios of mannuronic and guluronic acids). Only trace amounts of bacterial EPS could be isolated from leaves inoculated with a pathovar (pv. syringae) which does not induce the formation of lesions with a watersoaked appearance. Guluronic acid was either present in very low amounts or absent in the alginic acid preparations. All bacterial alginates were acetylated (7-11%). Levan (a fructan) was apparently not produced as an EPS in vivo by any of the pathogens tested.

References

  1. Anal Biochem. 1973 Aug;54(2):484-9 - PubMed
  2. J Bacteriol. 1986 Apr;166(1):66-71 - PubMed
  3. Appl Environ Microbiol. 1986 Sep;52(3):466-73 - PubMed
  4. Anal Biochem. 1978 Jun 15;87(1):206-10 - PubMed
  5. Anat Rec. 1971 Nov;171(3):347-68 - PubMed
  6. Annu Rev Microbiol. 1987;41:435-64 - PubMed
  7. Anal Biochem. 1981 Jul 1;114(2):294-8 - PubMed
  8. Plant Physiol. 1987 Oct;85(2):407-12 - PubMed
  9. Biochemistry. 1972 Apr 11;11(8):1383-8 - PubMed

Publication Types