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Bienfait HF, Lubberding HJ, Heutink P, et al. Rhizosphere Acidification by Iron Deficient Bean Plants: The Role of Trace Amounts of Divalent Metal Ions: A Study on Roots of Intact Plants with the Use of C- and P-NMR. Plant Physiol. 1989;90(1):359-64doi: 10.1104/pp.90.1.359.
Bienfait, H. F., Lubberding, H. J., Heutink, P., Lindner, L., Visser, J., Kaptein, R., & Dijkstra, K. (1989). Rhizosphere Acidification by Iron Deficient Bean Plants: The Role of Trace Amounts of Divalent Metal Ions: A Study on Roots of Intact Plants with the Use of C- and P-NMR. Plant physiology, 90(1), 359-64. https://doi.org/10.1104/pp.90.1.359
Bienfait, H F, et al. "Rhizosphere Acidification by Iron Deficient Bean Plants: The Role of Trace Amounts of Divalent Metal Ions: A Study on Roots of Intact Plants with the Use of C- and P-NMR." Plant physiology vol. 90,1 (1989): 359-64. doi: https://doi.org/10.1104/pp.90.1.359
Bienfait HF, Lubberding HJ, Heutink P, Lindner L, Visser J, Kaptein R, Dijkstra K. Rhizosphere Acidification by Iron Deficient Bean Plants: The Role of Trace Amounts of Divalent Metal Ions: A Study on Roots of Intact Plants with the Use of C- and P-NMR. Plant Physiol. 1989 May;90(1):359-64. doi: 10.1104/pp.90.1.359. PMID: 16666763; PMCID: PMC1061722.
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Plant Physiol. 1989 May;90(1):359-64. doi: 10.1104/pp.90.1.359.

Rhizosphere Acidification by Iron Deficient Bean Plants: The Role of Trace Amounts of Divalent Metal Ions: A Study on Roots of Intact Plants with the Use of C- and P-NMR.

Plant physiology

H F Bienfait, H J Lubberding, P Heutink, L Lindner, J Visser, R Kaptein, K Dijkstra

Affiliations

  1. Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.

PMID: 16666763 PMCID: PMC1061722 DOI: 10.1104/pp.90.1.359

Abstract

Rhizosphere acidification by Fe-deficient bean (Phaseolus vulgaris L.) plants was induced by trace amounts of divalent metal ions (Zn, Mn). The induction of this Fe-efficiency reaction was studied by (14)CO(2) and (11)CO(2) fixation experiments, and with (31)P-NMR on roots of whole plants. The starting and ending of an acidification cycle was closely coupled to parallel changes in CO(2) fixation, within the maximal resolution capacity of 20 min. (31)P-NMR experiments on intact root systems showed one peak which was ascribed to vacuolar free phosphate. At the onset of proton extrusion this peak shifted, indicating increase of pH in the cells. Proton extrusion was inhibited, with a lag period of 2 hours, by the protein synthesis inhibitors cycloheximide and hygromycin. It is assumed that Zn and Mn induce proton extrusion in Fe-deficient bean roots by activating the synthesis of a short-living polypeptide; the NMR data suggest a role for this peptide in the functioning of a proton pumping ATPase in the plasma membrane.

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