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Barrado E, Rodríguez JA, Castrillejo Y. Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks. Anal Bioanal Chem. 2006;385(7):1233-40doi: 10.1007/s00216-006-0356-y.
Barrado, E., Rodríguez, J. A., & Castrillejo, Y. (2006). Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks. Analytical and bioanalytical chemistry, 385(7), 1233-40. https://doi.org/10.1007/s00216-006-0356-y
Barrado, E, et al. "Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks." Analytical and bioanalytical chemistry vol. 385,7 (2006): 1233-40. doi: https://doi.org/10.1007/s00216-006-0356-y
Barrado E, Rodríguez JA, Castrillejo Y. Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks. Anal Bioanal Chem. 2006 Aug;385(7):1233-40. doi: 10.1007/s00216-006-0356-y. Epub 2006 Apr 22. PMID: 16633788.
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Anal Bioanal Chem. 2006 Aug;385(7):1233-40. doi: 10.1007/s00216-006-0356-y. Epub 2006 Apr 22.

Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks.

Analytical and bioanalytical chemistry

E Barrado, J A Rodríguez, Y Castrillejo

Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencias, Universidad de Valladolid, Paseo Prado de la Magdalena s/n, 47005, Valladolid, Spain. [email protected]

PMID: 16633788 DOI: 10.1007/s00216-006-0356-y

Abstract

A new chromatographic modality that does not require high pressures and also allows renewal of the stationary phase as desired is reported. The technique is based on a thin layer paramagnetic stationary phase (Fe3O4-SiO2) retained on the inner wall of a minicolumn through the action of an external magnetic field, which also plays an important role in separating the analytes. Accordingly, the name "renewable stationary phase liquid magnetochromatography", or RSP-LMC, has been proposed for it. The technique was used to separate and quantify the sugar substitute alpha-aspartame and its constituent amino acids (hydrolysis products), L-aspartic acid and L-phenylalanine, in diet fizzy soft drinks. When the results obtained for alpha-aspartame were compared with those obtained using HPLC as a reference method, no significant differences were observed. The system proposed is fully automated, making it an economic, competitive alternative to conventional methods of determining alpha-aspartame and its amino acid components.

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