Display options
Cite
Wong YS, McMichael RW, Lagarias JC. Properties of a polycation-stimulated protein kinase associated with purified Avena phytochrome. Plant Physiol. 1989;91(2):709-18doi: 10.1104/pp.91.2.709.
Wong, Y. S., McMichael, R. W., & Lagarias, J. C. (1989). Properties of a polycation-stimulated protein kinase associated with purified Avena phytochrome. Plant physiology, 91(2), 709-18. https://doi.org/10.1104/pp.91.2.709
Wong, Y S, et al. "Properties of a polycation-stimulated protein kinase associated with purified Avena phytochrome." Plant physiology vol. 91,2 (1989): 709-18. doi: https://doi.org/10.1104/pp.91.2.709
Wong YS, McMichael RW, Lagarias JC. Properties of a polycation-stimulated protein kinase associated with purified Avena phytochrome. Plant Physiol. 1989 Oct;91(2):709-18. doi: 10.1104/pp.91.2.709. PMID: 16667091; PMCID: PMC1062060.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1989 Oct;91(2):709-18. doi: 10.1104/pp.91.2.709.

Properties of a polycation-stimulated protein kinase associated with purified Avena phytochrome.

Plant physiology

Y S Wong, R W McMichael, J C Lagarias

Affiliations

  1. Department of Biochemistry and Biophysics, University of California, Davis, California 95616.

PMID: 16667091 PMCID: PMC1062060 DOI: 10.1104/pp.91.2.709

Abstract

ATP-dependent polycation-stimulated phosphorylation of highly purified phytochrome preparations from etiolated Avena seedlings has been reported previously (Y-S Wong, H-C Cheng, DA Walsh, JC Lagarias [1986] J Biol Chem 261: 12089-12097). In this study, we present a more detailed description of the properties of this protein kinase based on the analysis of over 30 different Avena phytochrome preparations. ATP-dependent phosphorylation of phytochrome was strongly stimulated by a wide range of polycationic molecules, including synthetic and natural polypeptides as well as nonpeptide cationic polymers. Many of the compounds known to stimulate other known protein kinases (i.e., cyclic nucleotides, Ca(2+), calmodulin, diacylglycerol, phospholipids) were either inhibitory or nonstimulatory. Among the polycations, histone H1, polylysine, and polybrene were the most effective, giving average stimulations of four- to sevenfold. Polycation-stimulated protein phosphorylation was inhibited by elevated ionic strength; of the salts examined, magnesium pyrophosphate was a particularly potent inhibitor of the kinase activity. MgATP was preferred as the phosphoryl donor to either MgGTP or magnesium pyrophosphate. The K(m) for MgATP was estimated to be 30 micromolar when histone H1 was used as a protein substrate. The Pr form of phytochrome was always a better substrate than the Pfr form regardless of the polycation present. Polylysine-stimulated, phytochrome(preparation)-dependent phosphorylation of purified maize phosphoenolpyruvate carboxylase was observed, as well as phosphorylation of a number of polypeptides in crude soluble protein extracts from etiolated Avena seedlings.

References

  1. CRC Crit Rev Biochem. 1982 Feb;12(2):133-86 - PubMed
  2. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  3. Plant Physiol. 1984 Jul;75(3):862-4 - PubMed
  4. Proc Natl Acad Sci U S A. 1989 May;86(10):3469-73 - PubMed
  5. J Biol Chem. 1986 Sep 15;261(26):12089-97 - PubMed
  6. J Biol Chem. 1985 Feb 25;260(4):2415-23 - PubMed
  7. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4250-4 - PubMed
  8. J Biol Chem. 1981 Feb 25;256(4):1681-8 - PubMed
  9. Arch Biochem Biophys. 1988 Mar;261(2):409-17 - PubMed
  10. Annu Rev Biochem. 1983;52:67-91 - PubMed
  11. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1408-11 - PubMed
  12. Biochemistry. 1980 Jan 22;19(2):390-4 - PubMed
  13. Proc Natl Acad Sci U S A. 1987 Feb;84(4):925-9 - PubMed
  14. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7388-91 - PubMed
  15. Cell. 1987 Sep 11;50(6):823-9 - PubMed
  16. Nature. 1982 Apr 15;296(5858):613-20 - PubMed
  17. J Biol Chem. 1983 Sep 25;258(18):11025-31 - PubMed
  18. Plant Physiol. 1988 May;87(1):195-200 - PubMed
  19. Photochem Photobiol. 1989 Mar;49(3):319-23 - PubMed
  20. Plant Physiol. 1983 Jan;71(1):150-5 - PubMed
  21. Anal Biochem. 1985 Oct;150(1):76-85 - PubMed

Publication Types