Display options
Cite
Altabella T, Chrispeels MJ. Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds. Plant Physiol. 1990;93(2):805-10doi: 10.1104/pp.93.2.805.
Altabella, T., & Chrispeels, M. J. (1990). Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds. Plant physiology, 93(2), 805-10. https://doi.org/10.1104/pp.93.2.805
Altabella, T, and Chrispeels, M J. "Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds." Plant physiology vol. 93,2 (1990): 805-10. doi: https://doi.org/10.1104/pp.93.2.805
Altabella T, Chrispeels MJ. Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds. Plant Physiol. 1990 Jun;93(2):805-10. doi: 10.1104/pp.93.2.805. PMID: 16667540; PMCID: PMC1062587.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1990 Jun;93(2):805-10. doi: 10.1104/pp.93.2.805.

Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds.

Plant physiology

T Altabella, M J Chrispeels

Affiliations

  1. Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0116.

PMID: 16667540 PMCID: PMC1062587 DOI: 10.1104/pp.93.2.805

Abstract

Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded by an already-identified lectin gene whose product is referred to as lectin-like-protein (LLP). We have now made a chimeric gene consisting of the coding sequence of the lectin gene that encodes LLP and the 5' and 3' flanking sequences of the lectin gene that encodes phytohemagglutinin-L. When this chimeric gene was expressed in transgenic tobacco (Nicotiana tabacum), we observed in the seeds a series of polypeptides (M(r) 10,000-18,000) that cross-react with antibodies to the bean alpha-amylase inhibitor. Most of these polypeptides bind to a pig pancreas alpha-amylase affinity column. An extract of the seeds of the transformed tobacco plants inhibits pig pancreas alpha-amylase activity as well as the alpha-amylase present in the midgut of Tenebrio molitor. We suggest that introduction of this lectin gene (to be called alphaai) into other leguminous plants may be a strategy to protect the seeds from the seed-eating larvae of Coleoptera.

References

  1. EMBO J. 1985 Dec 1;4(12):3047-53 - PubMed
  2. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7885-9 - PubMed
  3. Plant Physiol. 1972 Feb;49(2):187-9 - PubMed
  4. Proc Natl Acad Sci U S A. 1985 May;82(10):3320-4 - PubMed
  5. Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1371-7 - PubMed
  6. J Mol Appl Genet. 1984;2(5):447-53 - PubMed
  7. Plant Physiol. 1975 Aug;56(2):292-9 - PubMed
  8. EMBO J. 1985 Apr;4(4):883-9 - PubMed
  9. Plant Cell. 1989 Jan;1(1):95-104 - PubMed
  10. Science. 1988 Apr 8;240(4849):207-10 - PubMed
  11. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  12. Plant Physiol. 1979 Jun;63(6):1123-32 - PubMed
  13. Nucleic Acids Res. 1982 Dec 11;10(23):7819-28 - PubMed
  14. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8240-4 - PubMed
  15. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9871-5 - PubMed
  16. Science. 1976 May 21;192(4241):795-6 - PubMed
  17. EMBO J. 1987 Dec 1;6(12):3571-7 - PubMed

Publication Types