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Plant Physiol. 1990 Aug;93(4):1659-62. doi: 10.1104/pp.93.4.1659.

Salt-soluble lectins of corn grain.

Plant physiology

M Janković, M Cuperlović, L Hajduković

Affiliations

  1. Institute of Endocrinology, Immunology and Nutrition-INEP, Banatska 31b, 11080 Zemun, Yugoslavia.

PMID: 16667671 PMCID: PMC1062726 DOI: 10.1104/pp.93.4.1659

Abstract

Lectins extracted from corn (Zea mays L.) kernel with Tris-HCl buffer pH 7.5 were isolated from the crude extract by affinity chromatography on Sepharose 6B-N-acetyl-d-galactosamine and Sepharose 6B-methylalpha-d-mannoside, and also by lectin affinity chromatography using concanavalin A and Lens culinaris lectin as ligands. According to preferential monosaccharide specificity, salt-soluble lectins of corn seed comprise at least two distinct types: N-acetyl-d-galactosamine-interactive and mannose-interactive lectins. The extracted lectins are unstable, with a tendency to form aggregates during storage.

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