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Sellstedt A, Winship LJ. Acetylene, Not Ethylene, Inactivates the Uptake Hydrogenase of Actinorhizal Nodules during Acetylene Reduction Assays. Plant Physiol. 1990;94(1):91-4doi: 10.1104/pp.94.1.91.
Sellstedt, A., & Winship, L. J. (1990). Acetylene, Not Ethylene, Inactivates the Uptake Hydrogenase of Actinorhizal Nodules during Acetylene Reduction Assays. Plant physiology, 94(1), 91-4. https://doi.org/10.1104/pp.94.1.91
Sellstedt, A, and Winship, L J. "Acetylene, Not Ethylene, Inactivates the Uptake Hydrogenase of Actinorhizal Nodules during Acetylene Reduction Assays." Plant physiology vol. 94,1 (1990): 91-4. doi: https://doi.org/10.1104/pp.94.1.91
Sellstedt A, Winship LJ. Acetylene, Not Ethylene, Inactivates the Uptake Hydrogenase of Actinorhizal Nodules during Acetylene Reduction Assays. Plant Physiol. 1990 Sep;94(1):91-4. doi: 10.1104/pp.94.1.91. PMID: 16667724; PMCID: PMC1077194.
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Plant Physiol. 1990 Sep;94(1):91-4. doi: 10.1104/pp.94.1.91.

Acetylene, Not Ethylene, Inactivates the Uptake Hydrogenase of Actinorhizal Nodules during Acetylene Reduction Assays.

Plant physiology

A Sellstedt, L J Winship

Affiliations

  1. Department of Plant Physiology, University of Umeå, S-90187 UMEA, Sweden.

PMID: 16667724 PMCID: PMC1077194 DOI: 10.1104/pp.94.1.91

Abstract

Acetylene reduction assays were shown to inactivate uptake hydrogenase activity to different extents in one Casuarina and two Alnus symbioses. Inactivation was found to be caused by C(2)H(2) and not by C(2)H(4). Acetylene completely inactivated the hydrogenase activity of intact root systems of Alnus incana inoculated with Frankia strain Avcl1 in 90 minutes, as shown by a drop in the relative efficiency of nitrogenase from 1.0 to 0.73. The hydrogenase of Frankia preparations (containing vesicles) and of cell-free extracts (not containing vesicles) from the same symbiosis was much more susceptible to acetylene inactivation. Cell-free extracts lost all hydrogenase activity after 5 minutes of exposure to acetylene. The hydrogenase activity of intact root systems of Casuarina obesa was less sensitive to acetylene than that of root systems of A. incana, since the relative efficiency of nitrogenase changed only from 1.0 to 0.95 over 90 minutes. Frankia preparations and cell-free extracts of C. obesa still retained hydrogenase activity after a 10 minute-exposure to acetylene.

References

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