Display options
Cite
Hutchison KW, Sherman CD, Weber J, et al. Maturation in Larch : II. Effects of Age on Photosynthesis and Gene Expression in Developing Foliage. Plant Physiol. 1990;94(3):1308-15doi: 10.1104/pp.94.3.1308.
Hutchison, K. W., Sherman, C. D., Weber, J., Smith, S. S., Singer, P. B., & Greenwood, M. S. (1990). Maturation in Larch : II. Effects of Age on Photosynthesis and Gene Expression in Developing Foliage. Plant physiology, 94(3), 1308-15. https://doi.org/10.1104/pp.94.3.1308
Hutchison, K W, et al. "Maturation in Larch : II. Effects of Age on Photosynthesis and Gene Expression in Developing Foliage." Plant physiology vol. 94,3 (1990): 1308-15. doi: https://doi.org/10.1104/pp.94.3.1308
Hutchison KW, Sherman CD, Weber J, Smith SS, Singer PB, Greenwood MS. Maturation in Larch : II. Effects of Age on Photosynthesis and Gene Expression in Developing Foliage. Plant Physiol. 1990 Nov;94(3):1308-15. doi: 10.1104/pp.94.3.1308. PMID: 16667834; PMCID: PMC1077379.
Copy Download .nbib Format: NLM
Share it on

Plant Physiol. 1990 Nov;94(3):1308-15. doi: 10.1104/pp.94.3.1308.

Maturation in Larch : II. Effects of Age on Photosynthesis and Gene Expression in Developing Foliage.

Plant physiology

K W Hutchison, C D Sherman, J Weber, S S Smith, P B Singer, M S Greenwood

Affiliations

  1. Department of Biochemistry, University of Maine, Orono, Maine 04469.

PMID: 16667834 PMCID: PMC1077379 DOI: 10.1104/pp.94.3.1308

Abstract

The effect of maturation on the morphological and photosynthetic characteristics, as well as the expression of two genes involved in photosynthesis in the developing, current year foliage of Eastern larch (Larix laricina [Du Roi]) is described. These effects were observed on foliage during the third growing season after grafting of scions from trees of different ages onto 2 year old rootstock. Specific leaf weight (gram dry weight per square meter), leaf cross-sectional area (per square millimeter), and chlorophyll content (milligram per gram dry weight) all increase with increasing age in long shoot foliage from both indoor- and outdoor-grown trees. Net photosynthesis (NPS) (mole of CO(2) per square millimeter per second) increases with age on indoor- but not outdoor-grown trees. NPS also increases with increased chlorophyll content, but outdoor-grown scions of all ages had higher chlorophyll content, and chlorophyll does not appear to be limiting for NPS outdoors. To extend these studies of maturation-related differences in foliar morphology and physiology to the molecular genetic level, sequences were cloned from the cab and rbsS gene families of larch. Both cab and rbcS gene families are expressed in foliage but not in roots, and they are expressed in light-grown seedlings of larch but only at very low levels in dark-grown seedlings (~2% of light-grown seedlings). Steady-state cab mRNA levels are relatively higher (~40%) in newly expanding short shoot foliage from juvenile plants compared to mature plants. Unlike cab, the expression of the rbcS gene family did not seem to vary with age. These data show that the maturation-related changes in morphological and physiological phenotypes are associated with changes in gene expression. No causal relationship has been established, however. Indeed, we conclude that the faster growth of juvenile scions reported previously (MS Greenwood, CA Hopper, KW Hutchison [1989] Plant Physiol 90: 406-412) is not due to increased NPS or cab expression. Long shoot foliage is the dominant foliar type on young trees and its lower specific leaf weight will permit production of more photosynthetic surface area per unit of leaf biomass.

References

  1. Anal Biochem. 1983 Jul 1;132(1):6-13 - PubMed
  2. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
  3. Mol Cell Biol. 1985 Jun;5(6):1370-8 - PubMed
  4. Plant Mol Biol. 1990 Mar;14(3):287-96 - PubMed
  5. Plant Mol Biol. 1990 Feb;14(2):281-4 - PubMed
  6. Nucleic Acids Res. 1988 Dec 23;16(24):11830 - PubMed
  7. EMBO J. 1984 Aug;3(8):1671-9 - PubMed
  8. Plant Physiol. 1989 Jun;90(2):406-12 - PubMed
  9. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 - PubMed
  10. EMBO J. 1987 Dec 1;6(12):3593-9 - PubMed
  11. J Biol Chem. 1983 Feb 10;258(3):1399-402 - PubMed
  12. Science. 1986 May 30;232(4754):1106-12 - PubMed
  13. Gene. 1983 Nov;25(2-3):263-9 - PubMed
  14. Nucleic Acids Res. 1988 Dec 23;16(24):11829 - PubMed
  15. Nucleic Acids Res. 1986 May 27;14(10):4051-64 - PubMed
  16. J Biochem. 1987 Oct;102(4):673-6 - PubMed

Publication Types