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Pantoja O, Dainty J, Blumwald E. Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs. Plant Physiol. 1990;94(4):1788-94doi: 10.1104/pp.94.4.1788.
Pantoja, O., Dainty, J., & Blumwald, E. (1990). Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs. Plant physiology, 94(4), 1788-94. https://doi.org/10.1104/pp.94.4.1788
Pantoja, O, et al. "Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs." Plant physiology vol. 94,4 (1990): 1788-94. doi: https://doi.org/10.1104/pp.94.4.1788
Pantoja O, Dainty J, Blumwald E. Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs. Plant Physiol. 1990 Dec;94(4):1788-94. doi: 10.1104/pp.94.4.1788. PMID: 16667917; PMCID: PMC1077454.
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Plant Physiol. 1990 Dec;94(4):1788-94. doi: 10.1104/pp.94.4.1788.

Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs.

Plant physiology

O Pantoja, J Dainty, E Blumwald

Affiliations

  1. Centre for Plant Biotechnology, University of Toronto, Toronto, Ontario M5S 3B2, Canada.

PMID: 16667917 PMCID: PMC1077454 DOI: 10.1104/pp.94.4.1788

Abstract

The properties of the vacuolar membrane (tonoplast) ion channels of sugar beet (Beta vulgaries) cell cultures were studied using the patch-clamp technique. Tonoplast currents displayed inward rectification in the whole vacuole and isolated outside-out patch configurations and permeability ratios P(K+)/P(Na+) = 1 and P(K+)/P(Cl-) = 5. Amiloride and two of its analogs, 5-(N-methyl-N-isobutyl)-amiloride and benzamil, inhibitors of Na(+) channels in animal systems, blocked inward currents by reducing single-channel openings. Concentrations for 50% inhibition of vacuolar currents of 730 nanomolar, 130 nanomolar, and 1.5 micromolar for amiloride, benzamil, and 5-(N-methyl-N-isobutyl)-amiloride, respectively, were obtained from whole-vacuole recordings. The high inhibitory action (affinity) of amiloride and its analogs for the tonoplast cation channel suggests that these compounds could be used for the isolation and biochemical characterization of this protein.

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