Plant Physiol. 1991 Jan;95(1):335-6. doi: 10.1104/pp.95.1.335.
Plant physiology
M A Santana, L Villegas
PMID: 16667975 PMCID: PMC1077528 DOI: 10.1104/pp.95.1.335
Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration of a discontinuous distribution of protoplast isoelectric point from one species according to their surface potential.