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Logusch EW, Walker DM, McDonald JF, et al. Inhibition of plant glutamine synthetases by substituted phosphinothricins. Plant Physiol. 1991;95(4):1057-62doi: 10.1104/pp.95.4.1057.
Logusch, E. W., Walker, D. M., McDonald, J. F., & Franz, J. E. (1991). Inhibition of plant glutamine synthetases by substituted phosphinothricins. Plant physiology, 95(4), 1057-62. https://doi.org/10.1104/pp.95.4.1057
Logusch, E W, et al. "Inhibition of plant glutamine synthetases by substituted phosphinothricins." Plant physiology vol. 95,4 (1991): 1057-62. doi: https://doi.org/10.1104/pp.95.4.1057
Logusch EW, Walker DM, McDonald JF, Franz JE. Inhibition of plant glutamine synthetases by substituted phosphinothricins. Plant Physiol. 1991 Apr;95(4):1057-62. doi: 10.1104/pp.95.4.1057. PMID: 16668090; PMCID: PMC1077651.
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Plant Physiol. 1991 Apr;95(4):1057-62. doi: 10.1104/pp.95.4.1057.

Inhibition of plant glutamine synthetases by substituted phosphinothricins.

Plant physiology

E W Logusch, D M Walker, J F McDonald, J E Franz

Affiliations

  1. Monsanto Agricultural Company, A Unit of Monsanto Company, St. Louis, Missouri 63198.

PMID: 16668090 PMCID: PMC1077651 DOI: 10.1104/pp.95.4.1057

Abstract

Glutamine synthetase (GS) utilizes various substituted glutamic acids as substrates. We have used this information to design herbicidal alpha- and gamma-substituted analogs of phosphinothricin (l-2-amino-4-(hydroxymethylphosphinyl)butanoic acid, PPT), a naturally occurring GS inhibitor and a potent herbicide. The substituted phosphinothricins inhibit cytosolic sorghum GS(1) and chloroplastic GS(2) competitively versusl-glutamate, with K(i) values in the low micromolar range. At higher concentrations, these inhibitors inactivate glutamine synthetase, while dilution restores activity through enzyme-inhibitor dissociation. Herbicidal phosphinothricins exhibit low K(i) values and slow enzyme turnover, as described by reactivation characteristics. Both the GS(1) and GS(2) isoforms of plant glutamine synthetase are similarly inhibited by the phosphinothricins, consistent with the broad-spectrum herbicidal activity observed for PPT itself as well as other active compounds in this series.

References

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