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Morris RO, Jameson PE, Laloue M, et al. Rapid identification of cytokinins by an immunological method. Plant Physiol. 1991;95(4):1156-61doi: 10.1104/pp.95.4.1156.
Morris, R. O., Jameson, P. E., Laloue, M., & Morris, J. W. (1991). Rapid identification of cytokinins by an immunological method. Plant physiology, 95(4), 1156-61. https://doi.org/10.1104/pp.95.4.1156
Morris, R O, et al. "Rapid identification of cytokinins by an immunological method." Plant physiology vol. 95,4 (1991): 1156-61. doi: https://doi.org/10.1104/pp.95.4.1156
Morris RO, Jameson PE, Laloue M, Morris JW. Rapid identification of cytokinins by an immunological method. Plant Physiol. 1991 Apr;95(4):1156-61. doi: 10.1104/pp.95.4.1156. PMID: 16668105; PMCID: PMC1077666.
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Plant Physiol. 1991 Apr;95(4):1156-61. doi: 10.1104/pp.95.4.1156.

Rapid identification of cytokinins by an immunological method.

Plant physiology

R O Morris, P E Jameson, M Laloue, J W Morris

Affiliations

  1. Department of Biochemistry, University of Missouri-Columbia, Columbia, Missouri 65211.

PMID: 16668105 PMCID: PMC1077666 DOI: 10.1104/pp.95.4.1156

Abstract

A method for rapid identification of bacterial cytokinins has been developed in which cultures are fed [(3)H]adenine, the cytokinins (including (3)H-labeled cytokinins) are isolated by immunoaffinity chromatography, and analyzed by HPLC with on-line scintillation counting. Analysis of Agrobacterium tumefaciens strains showed that some produced primarily trans-zeatin, whereas others produced primarily trans-zeatin riboside. Pseudomonas syringae pv savastanoi produced mixtures of trans-zeatin, dihydrozeatin, 1''-methyl-trans-zeatin riboside, and other unknown cytokinin-like substances. Corynebacterium fascians, produced cis-zeatin, isopentenyladenine and isopentenyladenosine. The technique is designed for qualitative rather than quantitative studies and allows ready identification of bacterial cytokinins. It may also have utility in the study of plant cytokinins if adequate incorporation of label into cytokinin precursor pools can be achieved.

References

  1. Proc Natl Acad Sci U S A. 1966 Jul;56(1):52-9 - PubMed
  2. J Mol Biol. 1969 May 14;41(3):459-72 - PubMed
  3. Plant Physiol. 1987 Aug;84(4):982-6 - PubMed
  4. Biochem Biophys Res Commun. 1982 Aug;107(3):878-84 - PubMed
  5. Phytopathology. 1970 Jun;60(6):969-76 - PubMed
  6. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1560-6 - PubMed
  7. Plant Physiol. 1985 Jul;78(3):510-3 - PubMed
  8. Plant Physiol. 1986 Nov;82(3):742-7 - PubMed
  9. Biochem Biophys Res Commun. 1976 May 23;76(2):453-9 - PubMed
  10. Mol Plant Microbe Interact. 1988 Jul-Aug;1(6):235-42 - PubMed
  11. J Bacteriol. 1975 Jul;123(1):255-64 - PubMed
  12. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4776-80 - PubMed
  13. Proc Natl Acad Sci U S A. 1980 Jan;77(1):619-23 - PubMed

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