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Erion JL, Ballo B, May L, et al. Isolation and characterization of a tomato Acid phosphatase complementary DNA associated with nematode resistance. Plant Physiol. 1991;97(4):1462-9doi: 10.1104/pp.97.4.1462.
Erion, J. L., Ballo, B., May, L., Bussell, J., Fox, T., & Thomas, S. R. (1991). Isolation and characterization of a tomato Acid phosphatase complementary DNA associated with nematode resistance. Plant physiology, 97(4), 1462-9. https://doi.org/10.1104/pp.97.4.1462
Erion, J L, et al. "Isolation and characterization of a tomato Acid phosphatase complementary DNA associated with nematode resistance." Plant physiology vol. 97,4 (1991): 1462-9. doi: https://doi.org/10.1104/pp.97.4.1462
Erion JL, Ballo B, May L, Bussell J, Fox T, Thomas SR. Isolation and characterization of a tomato Acid phosphatase complementary DNA associated with nematode resistance. Plant Physiol. 1991 Dec;97(4):1462-9. doi: 10.1104/pp.97.4.1462. PMID: 16668572; PMCID: PMC1081187.
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Plant Physiol. 1991 Dec;97(4):1462-9. doi: 10.1104/pp.97.4.1462.

Isolation and characterization of a tomato Acid phosphatase complementary DNA associated with nematode resistance.

Plant physiology

J L Erion, B Ballo, L May, J Bussell, T Fox, S R Thomas

Affiliations

  1. Molecular Biology Group, Plant Cell Research Institute Incorporated, Dublin, California 94568.

PMID: 16668572 PMCID: PMC1081187 DOI: 10.1104/pp.97.4.1462

Abstract

The tomato (Lycopersicon esculentum) acid phosphatase-1 (Apase-1(1), EC 3.1.3.2) isozyme variant, genetically linked to the root-knot nematode resistance locus (Mi) on chromosome 6, has been purified by a rapid procedure from tomato cell suspension cultures. Peptide fragments of the purified enzyme were generated from trypsin and Lys-C endoprotease digests and separated by reverse-phase high-performance liquid chromatography. Amino acid sequences derived from the purified peptide fragments represented >50% of the total amino acid content of the protein and enabled the construction of degenerate oligonucleotide probes that were used to screen a tomato cell culture complementary DNA library. Clones corresponding to full-length coding sequences for Apase-1 have been isolated and sequenced. Southern blot analysis of DNA isolated from a number of tomato cultivars shows that the Apase-1(1) gene (aps1) is present at one copy per genome and that genotypes containing the aps1(1) allele have restriction fragment length polymorphisms that distinguish them from cultivars having the aps1(+) allele. Segregation analysis demonstrates that the restriction fragment length polymorphisms are associated with the aps1 locus. Tomato Apase-1(1) is also found to have significant homology at the amino acid sequence level to a class of vegetative storage proteins characterized in soybean.

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