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King DS, Bollenbacher WE, Borst DW, et al. The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro. Proc Natl Acad Sci U S A. 1974;71(3):793-6doi: 10.1073/pnas.71.3.793.
King, D. S., Bollenbacher, W. E., Borst, D. W., Vedeckis, W. V., O'connor, J. D., Ittycheriah, P. I., & Gilbert, L. I. (1974). The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro. Proceedings of the National Academy of Sciences of the United States of America, 71(3), 793-6. https://doi.org/10.1073/pnas.71.3.793
King, D S, et al. "The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro." Proceedings of the National Academy of Sciences of the United States of America vol. 71,3 (1974): 793-6. doi: https://doi.org/10.1073/pnas.71.3.793
King DS, Bollenbacher WE, Borst DW, Vedeckis WV, O'connor JD, Ittycheriah PI, Gilbert LI. The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro. Proc Natl Acad Sci U S A. 1974 Mar;71(3):793-6. doi: 10.1073/pnas.71.3.793. PMID: 16592146; PMCID: PMC388100.
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Proc Natl Acad Sci U S A. 1974 Mar;71(3):793-6. doi: 10.1073/pnas.71.3.793.

The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro.

Proceedings of the National Academy of Sciences of the United States of America

D S King, W E Bollenbacher, D W Borst, W V Vedeckis, J D O'connor, P I Ittycheriah, L I Gilbert

Affiliations

  1. Biochemistry Department, Zoëcon Corporation, Palo Alto, California 94304.

PMID: 16592146 PMCID: PMC388100 DOI: 10.1073/pnas.71.3.793

Abstract

Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like by radioimmunoassay. The prothoracic glands appear to be the sole source of this substance. The material was identified as alpha-ecdysone by thin-layer, gas-liquid, and high-resolution liquid chromatography and mass spectrometry. No other active moulting principles were present in the medium. It appears that the prothoracic gland secretes a prohormone, alpha-ecdysone, which is subsequently converted into the active moulting hormone, beta-ecdysone, in other insect tissues.

References

  1. Proc Natl Acad Sci U S A. 1973 May;70(5):1509-13 - PubMed
  2. Science. 1972 Oct 27;178(4059):418-9 - PubMed
  3. Science. 1972 Feb 18;175(4023):769-70 - PubMed
  4. Tex Rep Biol Med. 1954;12(4):1074-83 - PubMed
  5. Biol Bull. 1972 Apr;142(2):293-301 - PubMed
  6. Chem Ber. 1965 Jul;98(7):2394-402 - PubMed
  7. J Insect Physiol. 1971 Dec;17(12):2409-34 - PubMed
  8. J Insect Physiol. 1969 Feb;15(2):297-304 - PubMed
  9. Nature. 1969 Mar 8;221(5184):955-6 - PubMed
  10. Steroids. 1966 Nov;8(5):625-31 - PubMed
  11. Gen Comp Endocrinol. 1970 Aug;15(1):80-7 - PubMed

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