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Baianu IC, Critchley C, Govindjee, et al. NMR study of chloride ion interactions with thylakoid membranes. Proc Natl Acad Sci U S A. 1984;81(12):3713-7doi: 10.1073/pnas.81.12.3713.
Baianu, I. C., Critchley, C., Govindjee, & Gutowsky, H. S. (1984). NMR study of chloride ion interactions with thylakoid membranes. Proceedings of the National Academy of Sciences of the United States of America, 81(12), 3713-7. https://doi.org/10.1073/pnas.81.12.3713
Baianu, I C, et al. "NMR study of chloride ion interactions with thylakoid membranes." Proceedings of the National Academy of Sciences of the United States of America vol. 81,12 (1984): 3713-7. doi: https://doi.org/10.1073/pnas.81.12.3713
Baianu IC, Critchley C, Govindjee, Gutowsky HS. NMR study of chloride ion interactions with thylakoid membranes. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3713-7. doi: 10.1073/pnas.81.12.3713. PMID: 16593474; PMCID: PMC345289.
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Proc Natl Acad Sci U S A. 1984 Jun;81(12):3713-7. doi: 10.1073/pnas.81.12.3713.

NMR study of chloride ion interactions with thylakoid membranes.

Proceedings of the National Academy of Sciences of the United States of America

I C Baianu, C Critchley, Govindjee, H S Gutowsky

Affiliations

  1. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

PMID: 16593474 PMCID: PMC345289 DOI: 10.1073/pnas.81.12.3713

Abstract

The role of Cl(-) in photosynthetic O(2) evolution has been investigated by observing the (35)Cl NMR linewidth under a variety of conditions in aqueous suspensions of chloroplasts, primarily for the halophytes Avicennia germinans, Avicennia marina, and Aster tripolium but also for spinach. The line broadening shows there is weak, ionic binding of Cl(-) to thylakoids, the bound Cl(-) exchanging rapidly (>>10(4) sec(-1)) with free Cl(-) in solution. The binding is necessary for O(2) evolution to occur. Michaelis-Menten constants obtained from the Cl(-) dependence of the O(2) evolution rate are approximately 15-70 mM for the halophytes compared with 0.6 mM for spinach (0.5 mM with Br(-)). There appear to be two types of Cl(-) binding sites in halophytes, of which the stronger is the activator, at lower [Cl(-)], of O(2) evolution. The (35)Cl line broadening includes a nonspecific interaction, which becomes apparent at high Cl(-) concentrations (>/=0.5 M).

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