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Redfern CP. Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland. Proc Natl Acad Sci U S A. 1984;81(18):5643-7doi: 10.1073/pnas.81.18.5643.
Redfern, C. P. (1984). Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland. Proceedings of the National Academy of Sciences of the United States of America, 81(18), 5643-7. https://doi.org/10.1073/pnas.81.18.5643
Redfern, C P. "Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland." Proceedings of the National Academy of Sciences of the United States of America vol. 81,18 (1984): 5643-7. doi: https://doi.org/10.1073/pnas.81.18.5643
Redfern CP. Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5643-7. doi: 10.1073/pnas.81.18.5643. PMID: 16593512; PMCID: PMC391766.
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Proc Natl Acad Sci U S A. 1984 Sep;81(18):5643-7. doi: 10.1073/pnas.81.18.5643.

Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland.

Proceedings of the National Academy of Sciences of the United States of America

C P Redfern

Affiliations

  1. Department of Biochemistry, University of Liverpool, P. O. Box 147, Liverpool L69 3BX, England.

PMID: 16593512 PMCID: PMC391766 DOI: 10.1073/pnas.81.18.5643

Abstract

Ring glands or brain-ring gland complexes from third-instar Drosophila melanogaster larvae secreted ecdysone [(22R)-2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one] and two less polar ecdysteroids (LP1 and LP2) in vitro. Radioimmunoassay with different antisera indicated that LP1 and LP2 are side-chain-modified analogues of ecdysone. In high-performance liquid chromatography, the retention time of LP2 was equivalent to that of a precursor of makisterone A [(20R,22R)-2beta,3beta,14alpha,20,22,25-hexahydroxy-24-methyl-5beta-cholest-7-en-6-one] secreted by Dysdercus fasciatus prothoracic glands in vitro. LP2 was metabolized in vitro by the fat body of Drosophila larvae to a product with the characteristics of makisterone A when analyzed by gas chromatography/mass spectrometry (selected ion monitoring). Evidence was obtained for the presence of makisterone A in Drosophila larvae. These data suggest that LP2 is 20-deoxymakisterone A (24-methylecdysone) and that makisterone A could function as an additional moulting hormone in Drosophila, although 20-deoxymakisterone A production is apparently dependent on the sterol composition of the diet. LP1 has not been identified.

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