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Hartwig R, Schweiger M, Schweiger R, et al. Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia. Proc Natl Acad Sci U S A. 1985;82(20):6899-902doi: 10.1073/pnas.82.20.6899.
Hartwig, R., Schweiger, M., Schweiger, R., & Schweiger, H. G. (1985). Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia. Proceedings of the National Academy of Sciences of the United States of America, 82(20), 6899-902. https://doi.org/10.1073/pnas.82.20.6899
Hartwig, R, et al. "Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia." Proceedings of the National Academy of Sciences of the United States of America vol. 82,20 (1985): 6899-902. doi: https://doi.org/10.1073/pnas.82.20.6899
Hartwig R, Schweiger M, Schweiger R, Schweiger HG. Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6899-902. doi: 10.1073/pnas.82.20.6899. PMID: 16593618; PMCID: PMC390795.
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Proc Natl Acad Sci U S A. 1985 Oct;82(20):6899-902. doi: 10.1073/pnas.82.20.6899.

Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia.

Proceedings of the National Academy of Sciences of the United States of America

R Hartwig, M Schweiger, R Schweiger, H G Schweiger

Affiliations

  1. Max-Planck-Institut für Zellbiologie, Ladenburg bei Heidelberg, Federal Republic of Germany.

PMID: 16593618 PMCID: PMC390795 DOI: 10.1073/pnas.82.20.6899

Abstract

In the chloroplast fraction of the unicellular and uninucleate green alga Acetabularia, we have detected a M(r) approximately 230,000 protein (p230) whose synthesis exhibits a pronounced endogenous diurnal rhythm. As judged by scanning densitometry of fluorographs of NaDodSO(4)/polyacrylamide gels, the synthesis of other proteins in the same fraction was independent of the time in the cycle. The incorporation of [(35)S]methionine into p230 was completely inhibited by cycloheximide, whereas chloramphenicol had no effect. This strongly suggests that p230 is translated on 80S ribosomes. Eighthour periods of exposure to cycloheximide produced a shift in the phase of the oscillation of p230 synthesis. The results are consistent with the hypothesis that p230 is essential for expression of circadian rhythms in Acetabularia.

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