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Horsch RB, Klee HJ. Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process. Proc Natl Acad Sci U S A. 1986;83(12):4428-32doi: 10.1073/pnas.83.12.4428.
Horsch, R. B., & Klee, H. J. (1986). Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process. Proceedings of the National Academy of Sciences of the United States of America, 83(12), 4428-32. https://doi.org/10.1073/pnas.83.12.4428
Horsch, R B, and Klee, H J. "Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process." Proceedings of the National Academy of Sciences of the United States of America vol. 83,12 (1986): 4428-32. doi: https://doi.org/10.1073/pnas.83.12.4428
Horsch RB, Klee HJ. Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4428-32. doi: 10.1073/pnas.83.12.4428. PMID: 16593716; PMCID: PMC323746.
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Proc Natl Acad Sci U S A. 1986 Jun;83(12):4428-32. doi: 10.1073/pnas.83.12.4428.

Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.

Proceedings of the National Academy of Sciences of the United States of America

R B Horsch, H J Klee

Affiliations

  1. Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198.

PMID: 16593716 PMCID: PMC323746 DOI: 10.1073/pnas.83.12.4428

Abstract

We have developed a sensitive leaf disc transformation procedure for studying early and/or transient T-DNA expression during Agrobacterium tumefaciens-mediated transformation of plant cells. Using this system, we have examined the function of T-DNA border sequences on the early expression of T-DNA genes and on the stable integration of those genes in infected cells. Deletion of the right border from the T-DNA appears to permit transfer of T-DNA genes from the tumor-inducing (Ti) plasmid but greatly reduces the frequency of their stable integration. A binary vector has been constructed to permit examination of T-DNA border function in trans to the Ti plasmid. In this situation, a single T-DNA border is necessary for early expression of T-DNA genes and is sufficient for stable integration in any orientation.

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