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Dees C, Stringfellow D, Schultz RD. Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes. Theriogenology. 1984;21(4):661-75doi: 10.1016/0093-691x(84)90450-3.
Dees, C., Stringfellow, D., & Schultz, R. D. (1984). Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes. Theriogenology, 21(4), 661-75. https://doi.org/10.1016/0093-691x(84)90450-3
Dees, C, et al. "Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes." Theriogenology vol. 21,4 (1984): 661-75. doi: https://doi.org/10.1016/0093-691x(84)90450-3
Dees C, Stringfellow D, Schultz RD. Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes. Theriogenology. 1984 Apr;21(4):661-75. doi: 10.1016/0093-691x(84)90450-3. PMID: 16725915.
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Theriogenology. 1984 Apr;21(4):661-75. doi: 10.1016/0093-691x(84)90450-3.

Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes.

Theriogenology

C Dees, D Stringfellow, R D Schultz

Affiliations

  1. Dept. of Pathobiology, School of Veterinary Medicine University of Wisconsin-Madison 53715 USA.

PMID: 16725915 DOI: 10.1016/0093-691x(84)90450-3

Abstract

A commercially available follicle stimulating hormone preparation (FSH-P) was successfully incorporated into multilamellar vesicles (liposomes). Multilamellar liposomes were found to contain 9.39 +/- 1.14 mg FSH-P (n=4) per 100 mg phospholipid or approximately 19.0% of the original material used to form the liposomes. A 1% solution of Triton X-100 incubated with liposomes containing FSH-P for one-half hour at 37 degrees C released 33% of the entrapped FSH-P; more than 99% of the entrapped FSH-P was released when liposomes were incubated with a 2% solution of Triton X-100. Entrapment of FSH-P increased proportionally to the mole percentage of stearylamine used in liposome formation, suggesting that FSH-P is entrapped in the aqueous interstices of the cationic liposomes. Entrapment of FSH-P in stable liposomes suggests that these multilamellar vesicles may be useful as a FSH-P delivery vehicle used for the superovulation and embryo transfer of food animals.

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