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Segerson EC, Godfrey RW, Randel RD, et al. Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomized heifers. Theriogenology. 1986;25(5):639-52doi: 10.1016/0093-691x(86)90121-4.
Segerson, E. C., Godfrey, R. W., Randel, R. D., & Gunsett, F. C. (1986). Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomized heifers. Theriogenology, 25(5), 639-52. https://doi.org/10.1016/0093-691x(86)90121-4
Segerson, E C, et al. "Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomized heifers." Theriogenology vol. 25,5 (1986): 639-52. doi: https://doi.org/10.1016/0093-691x(86)90121-4
Segerson EC, Godfrey RW, Randel RD, Gunsett FC. Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomized heifers. Theriogenology. 1986 May;25(5):639-52. doi: 10.1016/0093-691x(86)90121-4. PMID: 16726154.
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Theriogenology. 1986 May;25(5):639-52. doi: 10.1016/0093-691x(86)90121-4.

Immunosuppressive activity of uterine luminal protein from steroid-treated ovariectomized heifers.

Theriogenology

E C Segerson, R W Godfrey, R D Randel, F C Gunsett

Affiliations

  1. Department of Animal Science, North Carolina A&T State University, Greensboro, NC 27411 USA.

PMID: 16726154 DOI: 10.1016/0093-691x(86)90121-4

Abstract

Uterine luminal protein (ULP) collected from ovariectomized steroid-treated crossbred heifers was tested for immunosuppressive activity in vitro. Heifers were allotted to treatment groups and for 16 d received daily injections of the following steroids or vehicle: Control (C, corn oil only, n=10); estradiol-17beta (E(2), 1.1 mug/kg body wt, n=10); progesterone (P(4), 2.2 mg/kg body wt, n=10); and E(2)+P(4) (1.1 mug + 2.2 mg/kg body wt, n=9). On Day 17, uterine flushings were collected, concentrated and quantitated for total ULP. ULP was tested for suppression of lymphocyte blastogenesis. For each experiment, 5 x 10(5) bovine lymphocytes were incubated with 0.4 mug of phytohemagglutinin (PHA) and ULP (25 to 400 mug ULP/ml) using standard culture conditions. At 48 h, 0.5 muCi of (3H) thymidine was added to cultures with cells harvested at 60 +/- 1 h by automation. Incorporated thymidine was measured by scintillation chromatography. Mean total ULP values for C-, E(2)-, P(4)- and E(2)+P(4)-treated groups were 4.7, 8.4, 13.6, and 25.5 mg, respectively (E(2)+P(4)>C and E(2), P<0.05). ULP from all treatment groups suppressed (P<0.0001) lymphocyte blastogenesis (thymidine incorporation) to PHA; however, suppression was greater (P<0.0001) for ULP from E(2)- and P(4)-than C-treated heifers at 100 and 200 mug ULP/ml. In conclusion, E(2) and P(4) injections enhanced immunosuppressive activity of ULP secretions.

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