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Theriogenology. 1990 Mar;33(3):741-7. doi: 10.1016/0093-691x(90)90551-4.

The effect of sucrose and trehalose on viability of one- and two-cell rabbit embryos.

Theriogenology

Z Smorag, Y Heyman, V Garnier, B Gajda

Affiliations

  1. Institute of Zootechnics Department of Animal Reproduction and AI 32-083 Balice/Krakow, Poland.

PMID: 16726770 DOI: 10.1016/0093-691x(90)90551-4

Abstract

The effect of sucrose and trehalose on the viability of one- and two-cell rabbit embryos was investigated. A significant decrease in the viability of one- and two-cell embryos exposed for 30 min. at 20 degrees C was observed. At 38 degrees C none of the two-cell embryos in a sucrose solution survived after 30 min exposure, while approximately 50% of the embryos survived in a trehalose solution. The cleveage rate in culture of two-cell embryos exposed both to 2.0 M or 1.45 M trehalose was significantly lower in comparison with the control group. However the survival rate after transfer of two-cell embryos exposed to 1.45 M trehalose solution at 20 degrees C remained the same as that of the control group.

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