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Kita M, Imai H. Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development. Theriogenology. 1993;40(2):357-64doi: 10.1016/0093-691x(93)90273-8.
Kita, M., & Imai, H. (1993). Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development. Theriogenology, 40(2), 357-64. https://doi.org/10.1016/0093-691x(93)90273-8
Kita, M, and Imai, H. "Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development." Theriogenology vol. 40,2 (1993): 357-64. doi: https://doi.org/10.1016/0093-691x(93)90273-8
Kita M, Imai H. Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development. Theriogenology. 1993 Aug;40(2):357-64. doi: 10.1016/0093-691x(93)90273-8. PMID: 16727321.
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Theriogenology. 1993 Aug;40(2):357-64. doi: 10.1016/0093-691x(93)90273-8.

Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development.

Theriogenology

M Kita, H Imai

Affiliations

  1. Ishikawa Prefectural Livestock Experiment Station, Oshimizu-cho, Hakui, Ishikawa, 929-13 Japan.

PMID: 16727321 DOI: 10.1016/0093-691x(93)90273-8

Abstract

The activity of hypoxanthine phosphoribosyltransferase (HPRT) was determined in the bovine embryo during early embryonic development. Microassay, using [(3)H] hypoxanthine, was improved to measure enzyme activity in the embryonic extract. This activity depended on the reaction time and the concentration of phosphorybosyl pyrophosphate (PRPP) in a reaction. mixture. Maximum activity was obtained at 4 hours of reaction time and at a concentration of 1 mM PRPP, but was much lower than the activity recorded in the mouse embryo. During early embryonic development, HPRT activity rapidly increased beyond the 8-cell stage. When distributions and activities of HPRT, adenine phosphorybosyltransferase (APRT), and the ratio of HPRT: APRT were examined in individual blastocysts, HPRT activity was broadly distributed, but it did not clearly show the bimodal distribution expected. Six of demi-embryos with high or low HPRT:APRT ratios were transferred to recipient cows from which 2 calves were obtained. Both offspring were of the sex predicted by the HPRT: APRT ratio. These results indicate that HPRT activity of bovine preimplantation embryos can be microassayed using radiolabeled hypoxanthine, and this assay could provide an alternative method for embryo sexing.

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