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Banks J, Eddie B, Schachter J, et al. Plaque formation by Chlamydia in L cells. Infect Immun. 1970;1(3):259-62doi: 10.1128/iai.1.3.259-262.1970.
Banks, J., Eddie, B., Schachter, J., & Meyer, K. F. (1970). Plaque formation by Chlamydia in L cells. Infection and immunity, 1(3), 259-62. https://doi.org/10.1128/iai.1.3.259-262.1970
Banks, J, et al. "Plaque formation by Chlamydia in L cells." Infection and immunity vol. 1,3 (1970): 259-62. doi: https://doi.org/10.1128/iai.1.3.259-262.1970
Banks J, Eddie B, Schachter J, Meyer KF. Plaque formation by Chlamydia in L cells. Infect Immun. 1970 Mar;1(3):259-62. doi: 10.1128/iai.1.3.259-262.1970. PMID: 16557725; PMCID: PMC415889.
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Infect Immun. 1970 Mar;1(3):259-62. doi: 10.1128/iai.1.3.259-262.1970.

Plaque formation by Chlamydia in L cells.

Infection and immunity

J Banks, B Eddie, J Schachter, K F Meyer

Affiliations

  1. The G. W. Hooper Foundation, University of California San Francisco Medical Center, San Francisco, California 94122.

PMID: 16557725 PMCID: PMC415889 DOI: 10.1128/iai.1.3.259-262.1970

Abstract

Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg ld(50) determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-conjunctivitis agents did not produce plaques. The plaque assay is highly sensitive, reproducible, and offers a potential tool for investigations requiring accurate measurement of small changes in chlamydial infectivity.

References

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