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Hopper DJ, Chapman PJ, Dagley S. Metabolism of l-Malate and d-Malate by a Species of Pseudomonas. J Bacteriol. 1970;104(3):1197-202doi: 10.1128/jb.104.3.1197-1202.1970.
Hopper, D. J., Chapman, P. J., & Dagley, S. (1970). Metabolism of l-Malate and d-Malate by a Species of Pseudomonas. Journal of bacteriology, 104(3), 1197-202. https://doi.org/10.1128/jb.104.3.1197-1202.1970
Hopper, D J, et al. "Metabolism of l-Malate and d-Malate by a Species of Pseudomonas." Journal of bacteriology vol. 104,3 (1970): 1197-202. doi: https://doi.org/10.1128/jb.104.3.1197-1202.1970
Hopper DJ, Chapman PJ, Dagley S. Metabolism of l-Malate and d-Malate by a Species of Pseudomonas. J Bacteriol. 1970 Dec;104(3):1197-202. doi: 10.1128/jb.104.3.1197-1202.1970. PMID: 16559093; PMCID: PMC248277.
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J Bacteriol. 1970 Dec;104(3):1197-202. doi: 10.1128/jb.104.3.1197-1202.1970.

Metabolism of l-Malate and d-Malate by a Species of Pseudomonas.

Journal of bacteriology

D J Hopper, P J Chapman, S Dagley

Affiliations

  1. Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55101.

PMID: 16559093 PMCID: PMC248277 DOI: 10.1128/jb.104.3.1197-1202.1970

Abstract

Extracts of a fluorescent species of Pseudomonas grown with m-cresol, degrade gentisic acid without isomerization of the ring-fission compound, maleylpyruvate, to give eventually d-malate and pyruvate. d-Malate is also a growth substrate. l-Malate but not d-malate is oxidized by a particulate enzyme not requiring nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP). NAD- or NADP-linked malate dehydrogenases are absent but cells contain an NADP-dependent l-malic enzyme. Exposure of cells to exogenous d-malate induces an NAD-dependent d-malic enzyme, not present when d-malate is formed endogenously. Succinate- or m-cresol-grown cells, containing no d-malic enzyme, rapidly oxidize d-malate in the presence of chloramphenicol at a concentration suffient to inhibit protein synthesis. An NADP-dependent cell-free system, prepared from succinate-grown cells which oxidized d-malate, is described.

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